• 1.5-4 mg Total RNA in 750 µl H2O
• pre-heat oligoTex beads to 37 C, vortex, set at Room Temp
• warm buffer OBB to 37 C, mix, place at Room Temp
• place 150 ul aliquot of OEB buffer at 70 C

• prepare 1 mg of Total RNA in 500 ul H20 or TE
• add 500 ul OBB
• add 55 ul OligoTex beads
• heat sample to 70 °C for 3 min.
• place at Room Temp, 10 min.
• spin 2 min., max speed
• remove sup
• re-suspend pellet in 400 ul OW2 by pipetting and move sup into a small spin column on a 1.5 ml tube
• spin 1 min, max speed
• remove eluate
• apply 400 ul OW2 and spin 1 min.
• transfer column to new tube
• apply 50 ul HOT OEB, pipet up and down to re-suspend resin
• spin 1 min.
• apply 50 ul HOT OEB, pipet up and down to re-suspend resin
• spin 1 min.
• place eluate on ice

yield is typically 5 - 15 ug