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| As a core facility, the Stowers Microscopy Center offers institute scientists access to a wide variety of well-maintained microscopes and imaging equipment. In addition, expert trainings are offered in the areas of widefield microscopy, confocal microscopy, specialized imaging techniques, and image processing and quantification. |
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Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities
Literature:
SMC Guidelines |
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 After submitting a SIRIS ticket requesting a widefield training, the process will take place in two parts. The first training will be on one of the Microscopy Center's widefield systems using standard training slides. It will be focused on gaining a foundational knowledge of microscopy. Next, a follow-up session will be scheduled to review the widefield training and determine imaging parameters specific to your sample and imaging needs. Each of the training sessions will last 2-3 hours with the goal of helping you become an excellent Microscopy Center user. |
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Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities
Web Links:
SIRIS
Literature:
SMC Guidelines
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 After submitting a SIRIS ticket requesting a confocal training, the process will take place in three parts. You will first be given a widefield training to gain a foundational knowledge of microscopy (unless you have already had a widefield training). Next, you will be trained on one of our general confocal systems using standard training slides. Finally, a follow-up session will be scheduled to review your knowledge of operating a microscope and optimize imaging parameters specific to your experiment. Prior to the follow up training we ask that users complete a follow up form. The purpose of this document is to familiarize your trainer with the goals of your experiment and to engage you in thinking about certain aspects of imaging that can improve the quality of your future data. Each of the training sessions will last 2-3 hours with the goal of helping you become an excellent Microscopy Center user. |
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Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities
Confocal Training Handout
Follow Up Training Form
Web Links:
SIRIS
Literature:
SMC Guidelines
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| Advanced trainings offered by the Stowers Microscopy Center include, but are not limited to; super-resolution, 2-photon, APD (Avalance Photo Diode detrators), spectral, multipositional, and live imaging, correlation spectroscopy, FRET, FLIM, FLIP, FRAP, photoactivation, and established methods of cuvette spectroscopy. These trainings can all be requested by submitting a training ticket for the Micrscopy Center in SIRIS. You can either note the specific training needed in the Additional Details field or check the appropriate box provided in the ticket. We are also glad to work with members of the institute to setup any experiments not included in the list above. |
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Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities
Web Links:
SIRIS
Literature:
SMC Guidelines
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SIRIS can be reached by typing siris into the address bar of your browser. Under the Microscopy Center tab select the type of assistance that you require and click "New Ticket". Fill out as much information as possible and use the "Additional Details" field to clarify the specifics of your request with a brief description.
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Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities
Web Links:
SIRIS
Literature:
SMC Guidelines
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LIMS can reached by typing limskc01 into the address bar of your browser. From this page you will be asked to login or create a new user account. After logging in 1) click on the Microscopy module tab. 2) Choose "New Reservation" and 3) select the microscope you want to book. After 4) designating what date and times you will be imaging 5) click "Make Reservation". 6) Fill out each field with the necessary information. Only fields with red text are mandatory. 7) Click "Create Reservation" and save the .ics file to your Outlook Calendar. If you choose to invite other members to the appointment they will receive a notification as well. Note that a microscope cannot be booked more than two weeks in advance and reservations may not be cancelled or shortened after the start time has passed. To directly link to a specific microscope's calendar you can also click "Place a Reservation" on our Instrumentation page.
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Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities
Web Links:
LIMS
Literature:
SMC Guidelines
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