Links

• General
• Microscopy
• Dyes
• Biology
• Chemistry
• Literature
• Language

• Wikipedia, the free encyclopedia that anyone can edit
• Wikipedia: Autocorrelation
• Wikipedia: Brownian Motion
• Wikipedia: Energy Levels
• dict.cc, an English - German dictionary
• Biophysical Society: Selected Topics in Biophysics
• Nobelprize.org: The laureates, their talks and lots of additional information
• Mathworld: Autocorrelation
• The Ophthalmic Photographers' Society
• Cold Spring Harbor Protocols
• photonics.com

• Molecular Expressions - Optical Microscopy Primer: Everything you want to know about microscopy
• Optical Microscopy Primer: Laser Scanning Confocal Microscopy
• Optical Microscopy Primer: Introduction to Fluorescence
• Microscopy Primer: Köhler Illumination
• Optical Microscopy Primer: Objectives
• Confocal List server: Very active discussion list
• Univeristy of Arizona: Microscopy & Imaging Resources on the WWW
• User group: Multi-Photon Laser Scanning Microscopy
• Nobelprize.org: Microscopes
• Carl Zeiss: Microscopy from the very beginning
• Nikon: MicroscopyU - The Source for Microscopy Education
• Olympus: Optical Microscopy for Biomedical Applications
• Institut Pasteur (PFID): Utilities for Imagery

Stowers Links:
Stowers Microscopy Center: Technology

• Invitrogen (Molecular Probes): Handbook
• Invitrogen (Molecular Probes): Spectra Viewer
• Carl Zeiss (Biorad): Fluorescence Dye Database
• Optical Microscopy Primer: Introduction to Fluorescence
• PubSpectra: A collection of absorption, fluorescence, transmission and reflectance spectral data

Fly

• Flybrain: Online Atlas of the Drosophila Nervous System
• FlyBase: Database of the Drosophila Genome
• FlyMove: Embryonic Development of Drosophila
• The Interactive Fly: A cyberspace guide to Drosophila development and metazoan evolution
• Atlas of Drosophila Development: by Volker Hartenstein
• Anatomical Atlas of Flies

Mouse

• Margatet J. Cook: The Anatomy of the Laboratory Mouse
• emap: Edinburgh Mouse Atlas Project

Neuroscience

• BrainMap: Online database of published functional neuroimaging experiments

• ChemInfo: Information about chemicals including NMR spectra, IR, HPLC chromatograms, mass, structure and links to suppliers

• PubMed: A service of the National Library of Medicine and the National Institute of Health
• Scopus: Commercial literature database, Stowers Institute has a subscription
• Biophysical Journal : Journal of the Biophysical Society

Stowers Links:
SIMR Comprehensive Journal List
Library Services

• dict.cc: English-German, German-English dictionary
• hyperdictionary: collection of multiple dictionaries

• 2nd Boston Workshop on Advanced TCSPC Techniques: Boston, Massachusetts, Monday September 22 - Tuesday September 23,2008
• 2008 IEEE International Conference on Image Processing: San Diego, California, Sunday October 12 - Wednesday October 15, 2008
• BiOS 2009 - SPIE Photonics West: San Jose, California, Saturday January 14 - Thursday January 19,2009
• Biophysical Society 53rd Annual Meeting: Boston, Massachusetts, Sunday February 28 - Wednesday March 4, 2009
• Focus on Microscopy 2009: Krakow, Poland, Sunday April 5 - Wednesday April 9, 2009

• Microscopy and Optics
• Accessories
• Lasers
• Dyes and Slide Preparation
• Biology and Biochemistry
• Tools

Microscopy:

• Carl Zeiss Microscopy & Imaging: Manufacturer of wide-field and confocal microscopes
• Carl Zeiss: Laser Scanning Microscopes
• Carl Zeiss: Filter Sets & Objectives
• FTP server confocal microscopy : Requires password
• Microscopy from the very beginning (Carl Zeiss)Download PDF
• The Clean Microscope (Carl Zeiss)Download PDF
• Carl Zeiss: Principles - Confocal Laser Scanning Microscopy Download PDF
• LSM Objectives (Carl Zeiss) Download PDF
• Carl Zeiss Imaging: Manufacturer of the cameras (Axiocam) and image processing software (Axiovision)
• Carl Zeiss Meditec: Manufacturer of slit lamps and other ophthalmologic instrumentation
• Leica Microsystems: Manufacturer of wide-field and confocal microscopes
• Leica: Confocal Microscopy
• Leica: Microscope Objectives
• Nikon: Manufacturer of wide-field and confocal microscopes
• Nikon: Biological Microscopes
• Olympus: Manufacturer of wide-field and confocal microscopes
• Olympus: Confocal Microscopy
• M. Abramowitz: Microscope Basics and Beyond (Olympus)Download PDF
• M. Davidson, M. Abramowitz: Optical Microscopy (Olympus) Download PDF
• Microscopy from the very beginning (Carl Zeiss)Download PDF
• Till Photonics: Manufacturer of the iMIC and TIRF solutions

Optical Filters:

• Carl Zeiss: Fluorescence Dye, Filter and Objective Databases
• Chroma: Manufacturer of optical filters.
• Chroma: Handbook of Optical Filters for Fluorescence Microscopy Download PDF
• Omega: Manufacturer of fluorescence filter sets.
• Semrock: Manufacturer of fluorescence filter sets.

Illumination:

• EXFO: Manufacturer of X-Cite fluorescence lamp.
• Technical Video.com: Manufacturer of fiber optic light scrambler, slide clean systems and heating/cooling stages.

Optical Components:

• Edmund Optics: Optical, opto-mechanical components and fiber optics
• Lighthouse Imaging Corporation: Medical Optical Engineering & Manufacturing
• Melles Griot: Optical, opto-mechanical components and lasers
• Navitar: Machine Vision
• Newport / Spectra-Physics : Optical, opto-mechanical components, lasers and spectrometer
• Ocular Instruments: Manufacturer of the mouse goniolens
• TTI Medical: Manufacturer of the universal video adapter

Fiber Optics:

• GrinTech: Gradient index optics technology
• Myriad Fiber Imaging: miniature fiber imaging systems
• Nanoptics: Custom Plastic Fiber Optic Specialists
• timbercon: Fiber Optic Products & Custom Solutions
• Schott: Introduction to fiber optic imaging
• Sumitomo Electric : Image Guide

Stowers Links:
Stowers Microscopy Center: Microscopy
Image Center: Instruments

Coverslips etc.

• Carolina: Samples mounted on microscope slides for teaching
• Evotec: Glass Bottom Microplates
• IBIDI: Chamber and channel slides for microscopy
• Mattek: Glass bottom culture pates and dishes
• NUNC: Plates, dishes and slides
• Klarmann Rulings, Inc: Stage micrometers and precision optical patterns
• Hecht-Assistent: Slides and coverslips
• Menzel-Gläser: Slides and coverslips
• Knittel-Glaeser: Slides and coverslips
• VWR: Slides and coverslips
• Willco Wells : Glass- and quartz bottom petri-dishes
• World Precission Instruments : Glass Bottom Dishes optimized for fluorescence microscopy
• Warner Instruments : Coverslip/Coverglass

Fluorescence Lifetime Systems (FLIM):

• Becker & Hickl : Manufacturer of fluorescence lifetime systems (FLIM).
• Becker & Hickl: Applications
• Wolfgang Becker: TCSPC Handbooks
• Wolfgang Becker: TCSP Handbook
• FLIM-LSM 510 Handbook
• Picoquant: Manufacturer of fluorescence lifetime (FLIM) and single photon counting systems
• ISS: Manufacturer of fluorescence lifetime (FLIM) and correlation (FCS) systems

Environmental Control:

• Bioptech : Live Cell Microscopy Environmental Control Systems
• ibidi Integrated BioDiagnostics: Slides with channels for flow control
• Life Imaging Services : Manufacturer of incubation chambers.
• Okolab: Manufacturer of incubation chambers.
• Solent Scientific: Manufacturer of incubation chambers.
• Warner Instruments: recording, imaging and perfusion chambers.
• Microscopy Live-Cell Imaging Chambers : more resources at Olympus FluoView Resource Center.

Detectors:

• Burle: supplier of specialized electron tubes and electro-optic products
• Perkin Elmer: Single Photon Counting Module
• Perkin Elmer: Single Photon-Counting Technology, Download PDF
• Radiation Monitoring Devices: Large area APDs.

Stowers Links:
Stowers Microscopy Center: Technology

• Coherent: Manufacturer of lasers, including Chameleon for two-photon excitation
• Spectra Physics: Manufacturer of lasers, including Mai Tai for two-photon excitation
• Del Mar Photonics: Manufacturer of components for ultra-fast lasers (including lasers)
• Prairie Technologies : Manufacturer of microscope components, including laser launches
• Spectral Applied Research: Manufacturer of optical components, including LMM5 laser merge module

Stower's Links:
Stowers Microscopy Center: Non-Linear Optics

• Invitrogen (was Molecular Probes) Manufacturer of Alexa and lots of other dyes, very helpful handbook and additional resources.
• Invitrogen (Molecular Probes): Handbook
• Invitrogen (Molecular Probes): Spectra Viewer
• Bangs Laboratories: Fluorescent and other microspheres
• bistatus: Manufacturer of DNA probe DRAQ5 and mounting media CyGEL
• Duke Scientific : Microspheres and particles
• Optical Imaging: Voltage sensitive dyes
• Polysciences: Microspheres, slides and polymerproducts
• Sherotech: Fluorescent and other particles
• Seradyn: Fluorescent and other particles

Stowers Links:
Histology Facility

• Genlantis: Primary cells and gene delivery products.

• Fine Science Tools:Good forceps, scalpels, scissors etc.
• World Precission Instruments : Tools for microscopy and laboratory use

• Presentations
• Literature
• Software
• Manuals

• Winfried Wiegraebe: Don't Waste Photons: Friday Science Club presentation March 2006 (View as Powerpoint presentation, click on slides to see animation)
• Winfried Wiegraebe: Basics in Microscopy: Transmitted Light (View as Powerpoint presentation)
• Winfried Wiegraebe: Art And Neuroscience (View as Powerpoint presentation)

Stowers Links:
Stowers Microscopy Center: Seminars

PDF downloads in red for internal use only. Sorry.

Microscopy:

• M. Abramowitz: Microscope Basics and Beyond (Olympus)Download PDF
• M. Davidson, M. Abramowitz: Optical Microscopy (Olympus) Download PDF
• Microscopy from the very beginning (Carl Zeiss)Download PDF
• The Clean Microscope (Carl Zeiss)Download PDF
• LSM Objectives (Carl Zeiss) Download PDF
• Smith, R. S., D. Korb, et al. (2002). "A goniolens for clinical monitoring of the mouse iridocorneal angle and optic nerve." Molecular Vision 8: 26-31.Download PDF

Confocal Microscopy:

• Carl Zeiss: Principles - Confocal Laser Scanning Microscopy Download PDF

Fluorescence:

• Lichtman, J. W. and J.-A. Conchello (2005). "Fluorescence microscopy." 2(12): 910-919. Download PDF
• Shaner, N. C., P. A. Steinbach, et al. (2005). "A guide to choosing fluorescent proteins." Nature Methods 2(12): 905-909. Download PDF

Optical Filters:

• Chroma: Handbook of Optical Filters for Fluorescence Microscopy Download PDF

Fluorescence Lifetime Imaging Microscopy (FLIM):

• Wolfgang Becker: TCSPC Handbooks
• Wolfgang Becker: TCSP Handbook
• FLIM-LSM 510 Handbook

Fluorescence Correlation Spectroscopy (FCS):

• Elson, E. L. and D. Magde (1974). "Fluorescence correlation spectroscopy. I. Conceptual basis and theory." Biopolymers 13(1): 1-27. Download PDF
• Magde, D. and E. L. Elson (1974). "Fluorescence Correlation Spectroscopy. II. An Experimental Realization." Biopolymers 13: 29-61.Download PDF
• P. Schwille, E. Haustein: Fluorescence Correlation Spectroscopy Download PDF
• Wiegraebe, W. (2000). "Fluorescence correlation microscopy: Probing molecular interactions inside living cells." Am. Lab. Sept.: 44-47. Download PDF
• Dancing Molecules. Download PDF
• Carl Zeiss: Applications Manual LSM 510 - ConfoCor 2 Application Download PDF

Detectors:

• Perkin Elmer: Single Photon-Counting Technology, Download PDF

• Carl Zeiss LSM Image Browser: Viewing, comparing, sorting and printing LSM 5 images.
• Download AxioVision LE: free image browser for Carl Zeiss Axiovision images
• FCS Viewer : Allows to view ConfoCor 2 and 3 raw data files

Stowers Links:
Stowers BioInformatics Center

• Carl Zeiss ConfoCor 3: Manual for the FCS part of our LSM 510-DEV system. Chapter 4 describes the operation of the ConfoCor 3. Chapter 5 summarizes the operation of the FCS as well as the LSM part.
• Carl Zeiss FCS Application Handbook: Information about how to perform FCS measurements. Very helpful.
• Carl Zeiss ConfoCor 2 Raw Data Format : Describes how the FCS raw data (the photon counts per time) are stored. Note: a new format is introduced with software Rel. 4.0 (the release we are using at the LSM 510-DEV system.
• Carl Zeiss ConfoCor 3 Raw Data Format : Shows how the FCS raw data (the photon counts per time) are stored with software Rel. 4.0 (the release we are using at the LSM 510-DEV system.
• Carl Zeiss LSM 510 META Rel. 3.5: Manual for system LSM 510-VIS and LSM 510-NLO. Chapter 3 gives a nice introduction into confocal microscopy. Chapter 4 is the main manual. Chapter 7 describes the software package 3D for LSM. Chapter 9 is a very good introduction into how to use a multi-photon (NLO) confocal microscope.
• Carl Zeiss LSM 5 PASCAL Rel. 3.5: Chapter 3 gives a nice introduction into confocal microscopy. Chapter 4 is the main manual. Chapter 6 describes the software package 3D for LSM. The last chapter summarizes the operation of the system.
• Coherent Chameleon Ultra : NIR laser used for two-photon excitation at LSM 510-DEV. Similar to laser at the LSM 510-NLO. Chapter 7 is a good introduction into the physics of pulsed lasers.
• Wolfgang Becker: TCSP Handbook
• FLIM-LSM 510 Handbook
• ACCU-Beam User Manual: Manual for the ACCU-Beam video adapter used together with the slit-lamp for mouse eye imaging. Download PDF

• Public Pages
• For Internal Use Only

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• Kemper Museum of Contemporary Art
• The Nelson-Atkins Museum of Art
• Crossroads District: Lots of art galleries, famous for its first Fridays.
• Review: Local Art Magazine, Studio space

Stowers Links:
Stowers Microscopy Center: People

• Friends of Chamber Music: Performances by international acclaimed musicians.
• The Lyric Opera of Kansas City
• Kansas City Symphony
• Blue Room: Jazz Club at the American Jazz Museum.
• Jardine's: Jazz Club and restaurant, mostly local artists.
• The Majestic: Jazz Club and steak house, mostly local artists.

• Kansas City (MO): Official web site of City of Kansas City
• NPR Kansas City
• Recycling at Kansas City (MO): and Map of Locations
• University of Missouri-Kansas City
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