The Stowers Institute For Medical Research: Microscopy Center

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Microscopy Center
Head: Winfried Wiegraebe

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Winfried Wiegraebe

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Winfried Wiegraebe

Winfried Wiegraebe
Head, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4415
Fax: (816) 926-2088
email: wiw@stowers.org

Winfried Wiegraebe CV

2005 - present
 
2008 - present

Head, Stowers Microscopy Center
Stowers Institute for Medical Research

2005 - 2008

Director, Advanced Instrumentation & Physics
Stowers Institute for Medical Research

2001 - 2005

Field Specialist Laser Scanning Microscopy and Fluorescence Correlation Spectroscopy
Carl Zeiss MicroImaging, Inc.
One Zeiss Drive
Thornwood , NY 10594
USA

1999 - 2001

Product Manager Fluorescence Correlation Spectroscopy
Carl Zeiss Jena, GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany

1996 - 1999

R&D Team Leader Scanning Near Field Optical Microscopy
Carl Zeiss Jena, GmbH
Carl-Zeiss-Promenade 10
07745 Jena
Germany

1994 - 1996

Post Doc: AFM and Humidity based STM on Microtubules
Institute for Molecular Biology
Research Group of Molecular Cytology - Electron Microscopy
Prof. Unger
Beutenbergstr. 11
07745 Jena
Germany

1989 - 1994

PhD - Biophysics: "A multifunctional Scanning Force Microscope for Biological Applications"
Max-Planck-Institute for Biochemistry
Dept. of Molecular Structural Biology, Prof. Baumeister
Am Klopferspitz 18
82152 Martinsried
Germany

1988 - 1989

Diploma thesis: "Scanning Tunneling Microscopy Based Analysis of Biological Specimen"
Max-Planck-Institute for Biochemistry
Dept. of Molecular Structural Biology, Prof. Baumeister
Am Klopferspitz 18
82152 Martinsried
Germany

1982 - 1988

Study of "Biophysics" and "Machine Tools and Industrial Management"
Technical University Munich
Arcisstr. 21
80290 Munich
Germany

Stowers Microscopy Publications

Lange, J., Wood, C., Marshall, W., Maddera, L., Yu, Q., Bradford, W., Slaughter, B., Unruh, J., and W. Wiegraebe (2013). "Correction of bleaching artifacts in high content fluorescence correlation spectroscopy (HCS-FCS) data." Proc. SPIE8590, Single Molecule Spectroscopy and Superresolution Imaging IV, 859006.

Ma L., Qiu Q., Gradwohl S., Scott A., Yu E.Q., Alexander R., Wiegraebe W., Yu C.R. (2012). "Distributed Representation of Chemical Features and Tunotopic Organization of Glomeruli in the Mouse Olfactory Bulb." PNAS 109 (14): 5481-5486.

Hao, Y., Xu, N., Schaeferl, L., Kannan, K., Zhang, Y., Florens, L., Seidel, C., Washburn, M., Wiegraebe, W., and Mak, H.Y. (2011). "Nuclear cGMP-Dependent Kinase Regulates Gene Expression via Activity-Dependent Recruitment of a Conversed Histone Deacetylase Complex." PLoS Genetics 7:e1002065.

Gardner, J.M., Smoyer, C.J., Stensrud, E.S., Alexander, R., Gogol, M., Wiegraebe, W., and Jaspersen, S.L. (2011). "Targeting of the SUN protein Mps3 to the inner nuclear membrane by the histone variant H2A.Z." Journal of Cell Biology 193(3): 489-507.

Wood, C., Huff, J., Marshall, W., Yu, Q., Unruh, J., Slaughter, B., and W. Wiegraebe (2011). "Fluorescence correlation spectroscopy as tool for high-content-screening in yeast (HCS-FCS)," Proceedings of SPIE Vol. 7905, 79050H.

Lutes, A. A., W. B. Neaves, D. P. Baumann, W. Wiegraebe and P. Baumann (2010). "Sister chromosome pairing maintains heterozygosity in parthenogenetic lizards." Nature 464(7286): 283-286.

Xie, Y., T. Yin, W. Wiegraebe, X. He, D. Miller, D. Stark, K. Perko, R. Alexander, J. W. Schwartz, J. Grindley, J. Park, J. Haug, J. Wunderlich, H. Li, S. Zhang, T. Johnson, R. Feldman and L. Li (2009). "Detection of functional haematopoietic stem cell niche using real-time imaging." Nature 457: 97-101.

W. Wiegraebe, Y. Xie, T. Yin, D. Stark, K. Perko, R. Alexander, J. Schwartz, X. C. He and L. Li (2009). "Two-Photon Microscopy and Spectral Detection for Ex Vivo Imaging of Individual Stem Cells." Nature protocols.

Slaughter, B. D., J. M. Huff, W. Wiegraebe, J. W. Schwartz and R. Li (2008). "SAM domain-based protein oligomerization observed by live-cell fluorescence fluctuation spectroscopy." PLoS ONE 3(4).

Aulehla, A., W. Wiegraebe, V. Baubet, M. B. Wahl, C. Deng, M. Taketo, M. Lewandoski and O. Pourquie (2008). "A β-catenin gradient links the clock and wavefront systems in mouse embryo segmentation." Nature Cell Biology 10(2): 186-193.

Other Publications

Rigler, R. and W. Wiegraebe (2001). "Highlight: Fluorescence correlation spectroscopy." Biological Chemistry 382(3): 353-354.

W. Wiegraebe (2000). "Fluorescence correlation microscopy: Probing molecular interactions inside living cells." American Laboratory 32(18): 44-47.

W.Wiegräbe, S. M., H.Dittmar, K.O.Greulich, S.Häfner, M.Hildebrandt, M.Kittler, B.Lochner, E.Unger (1997). "Scanning near field optical microscope: A method for investigating chromosomes." Surface and Interface Analysis 25: 510-513.

W. Wiegräbe, H. F. K., H. Eberhard, R. Gatz, T. Hartmann, M. Heim, Ch. Lorek, and R. Guckenberger (1995). "A multifunctional scanning force microscope for biological applications." Rev. Sci. Instrum. 66(8): 4124-4129.

Knapp, H. F., W. Wiegrabe, M. Heim, R. Eschrich and R. Guckenberger (1995). "Atomic force microscope measurements and manipulation of Langmuir-Blodgett films with modified tips." Biophys J 69: 708-15.

Heim, M., G. Cevc, R. Guckenberger, H. F. Knapp and W. Wiegrabe (1995). "Lateral electrical conductivity of mica-supported lipid bilayer membranes measured by scanning tunneling microscopy." Biophys J 69: 489-97.

Guckenberger, R., M. Heim, G. Cevc, H. F. Knapp, W. Wiegräbe and A. Hillebrand (1994). "Scanning tunneling microscopy of insulators and biological specimens based on lateral conductivity of ultrathin water films." Science 266: 1538-40.

Hartmann, T., R. Gatz, W. Wiegräbe, R. Kramer, A. Hillebrand, K. Lieberman, W. Baumeister and R. Guckenberger (1993). A Scanning Near-Field Optical Microscope (SNOM) for Biological Applications. Near Field Optics. D. W. Pohl and D. Courjon, Kluwer Academic Publishers: 35-44.

Hillebrand, A., T. Scheybani, W. Wiegräbe and R. Guckenberger (1992). "Reduction by ion-milling of surface corrugation of thin amorphous carbon films for biological specimen substrates in scanning tunneling microscopy." Ultramicroscopy 42-44: 1255-1259.

Guckenberger, R., T. Hartmann, W. Wiegräbe and W. Baumeister (1992). The Scanning Tunneling Microscope in Biology. Scanning Tunneling Microscopy II. R. Wiesendanger and H.-J. Güntherodt. Berlin Heidelberg, Springer-Verlag. 28: 51-98.

W. Wiegräbe., M. Nonnenmacher, R. Guckenberger and O. Wolter (1991). "Atomic force microscopy of a hydrated bacterial surface protein." J Microsc 163 ( Pt 1): 79-84.

Guckenberger, R., B. Hacker, T. Hartmann, T. Scheybani, Z. Wang, W. Wiegräbe and W. Baumeister (1991). "Imaging of Uncoated Purple Membrane by Scanning Tunneling Microscopy." J.Vac.Sci.Technol.B 9: 1227-1230.

Guckenberger, R., W. Wiegräbe, A. Hillebrand, T. Hartmann, Z. Wang and W. Baumeister (1989). "Scanning Tunneling Microscopy of a Hydrated Bacterial Surface Protein." Ultramicroscopy 31: 327-332.

Guckenberger, R., W. Wiegrabe and W. Baumeister (1988). "Scanning tunneling microscopy of biomacromolecules." J Microsc 152 ( Pt 3): 795-802.

Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities

Web Links:
Carl Zeiss
Carl Zeiss: Confocal Microscopy
Carl Zeiss: Fluorescence Correlation Spectroscopy
Institute for Molecular Biotechnology (Jena), renamed to "Leibniz Institute for Age Research - Fritz Lipmann Institute"
Jena, in German
Max Planck Institute of Biochemistry
MPI of Biochemistry: Molecular Structural Biology
Stowers Institute: Google map
Technische Universität München, in German
TUM: Biophysics
Neustadt an der Weinstraße
Neustadt an der Weinstraße: Google Map

Winfried Top
Richard Members

Richard Alexander

Winfried Top
Steven Members
Richard Alexander

Richard Alexander
Research Specialist II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4444
Fax: (816) 926-2088
email: rla@stowers.org

2008 - present
 
2008 - present
Senior Research Technician, Stowers Microscopy Center
Stowers Institute for Medical Research
2007 - 2008
Solution Consultant
Cerner Corporation
Kansas City, MO
USA
2005 - 2007
Business Analyst
Cerner Corporation
Kansas City, MO
USA
2002 - 2005
M.S. Physics
University of Kansas
Lawrence, KS
USA
1998 - 2002
B.S. Physics
Oklahoma City University
Oklahoma City, OK
USA
Stowers Microscopy Publications
 

Ghosh, S., Gardner, J.M., Smoyer, C.J., Friederichs, J.M., Unruh, J.R., Slaughter, B.D., Alexander, R., Chisholm, R.D., Lee, K.K., Workman, J.L., Jaspersen, S.L. (2012). "Acetylation of the SUN protein Mps3 by Eco1 regulates its function in nuclear organization". Mol Biol Cell. 23(13): 2546-2559.

Ma, L., Qiu, Q., Gradwohl, S., Scott, A., Yu, E.Q., Alexander, R., Wiegraebe, W., Yu, C.R. (2012). "Distributed representation of chemical features and tunotopic organization of glomeruli in the mouse olfactory bulb". Proc Acad Sci USA. 109(14): 5481-5486.

Das, A., Slaughter, B.D., Unruh, J.R., Bradford, W.D., Alexander, R., Rubinstein, B., Li, R.(2012). "Flippase-mediated phospholipid asymmetry promotes fast Cdc42 recycling in dynamic maintenance of cell polarity". Nat Cell Biol. 14(3): 304-10.

Ma, L., Qiua, Q., Gradwohl, S., Scott, A., Yu, E.Q., Alexander, R., Wiebraebe, W., Yu, R.C. (2012). "Reply to Yablonka et al.: Parity in data processing is essential in correlation analyses". PNAS. 109(43): 2918-2919.

Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities

Web Links:
Cerner Corporation
University of Kansas
Oklahoma City University

Richard Top

Steven

Members

Steven Hoffman

Richard Top
Amanda Members
Steven Hoffman

Steven Hoffman
Group Head, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 830-8021
Fax: (816) 926-4691
email: sjh@stowers.org

2009 - present
 
2011 - present
Group Head, Stowers Institute for Medical Research
Stowers Institute for Medical Research
2009 - 2011
Senior Research Specialist, Stowers Microscopy Center
Stowers Institute for Medical Research
2007 - 2009
Research Specialist II, Stowers Microscopy Center
Stowers Institute for Medical Research
2002 - 2007
Software Engineer
Cerner Corporation
1992 - 2001
1988 - 1992
Associate Psychologist
Midwest Research Institute
Other Publications  

Cook, M. R., M. M. Gerkovich, S. J. Hoffman, and F. J. McClernon (1995). “Gender Differences in the Effects of Smoking on the EEG,” Psychophysiology, 32 (Supplement 1), S25.
 
Cook, M. R., M. M. Gerkovich, S. J. Hoffman, and F. J. McClernon (1995). “The Interaction of Smoking Effects and Individual Differences on Cardiac and EEG Activity,” Psychophysiology, 32 (Supplement 1), S34.
 
Cook M. R., M. M. Gerkovich, S. J. Hoffman, F. J. McClernon, H. D. Cohen, K. L. Oakleaf, K. A. O'Connell (1995). “Smoking and EEG power spectra: effects of differences in arousal seeking,” International journal of psychophysiology 19(3):247-56.
 
McClernon, F. J., M. R. Cook, M. M. Gerkovich, S. J. Hoffman, and J. C. Beaty, Jr., “Effects of Respiratory Maneuvers on End Tidal CO2 and the EEG of Smokers,” Psychophysiology, 32 (Supplement 1), S52 (1995).
 
Cook, M. R., M. M. Gerkovich, S. J. Hoffman, F. J. McClernon, and K. A. O’Connell (1996). “Effects of Smoking and Telic/Paratelic Dominance on the CNV,” International Journal of Psychophysiology, 23:101-110.
 
Gerkovich, M. M., M. R. Cook, S. J. Hoffman, and K. A. O’Connell (1998). “Individual Differences in Cardiac and EMG Activity After Smoking,” Personality and Individual Differences, 25, 353-364.
 
Graham, C., M. R. Cook, H. D. Cohen, D. W. Riffle, S. Hoffman, and M. M.
Gerkovich (1999). “Human Exposure to 60-Hz Magnetic Fields: Neurophysiological Effects,” International Journal of Psychophysiology, 33, 169-175.

Cook, M.R., M.M. Gerkovich, C. Graham, S.J. Hoffman, R.C. Peterson (2003). “Effects of the nicotine patch on performance during the first week of smoking cessation,” Nicotine and Tobacco Research, 5 (2), pp. 169-180.

Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities

Web Links:
Cerner Corporation
Midwest Research Institute

Steven Top
Amanda Members

Amanda Kroesen

Steven Top
Jeffrey Members
Amanda Kroesen

Amanda Kroesen
Laboratory Assistant, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4364
Fax: (816) 926-4691
email: aec@stowers.org

2006- present
 
2008 - present
Laboratory Assistant, Stowers Microscopy Center
Stowers Institute for Medical Research
2006 - 2008
Research Technician III, Stowers Microscopy Center
Stowers Institute for Medical Research
  M.S. Chemistry
University of Colorado
Boulder, CO
USA
  B.S. Chemistry
Truman State University
Kirksville, MO
USA
Stowers Microscopy Publications
 
Y. Nakajima, Meyer, J., Kroesen, A., McKinney, S., Gibson, M.C. (2013). "Epithelial junctions maintain tissue by directing planar spindle orientation". Nature. 500(7462): 359-362.

Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities

Web Links:
University of Colorado
Truman State University

Steven Top
Jeffrey Members

Jeffrey Lange

Amanda Top
Lucinda Members

Jeffrey Lange
Research Specialist I, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4331
Fax: (816) 926-4691
email: jjl@stowers.org

2011 - present
 
2011 - 2014
Research Specialist I, Stpwers Microscopy Center
Stowers Institute for Medical Research
2009 - 2011
Postdoctoral Research Scholar
Washington University
St. Louis, MO
USA
2004 - 2009
Ph.D. Analytical Chemistry
Kansas State University
Manhattan, KS
USA
2000 - 2004
B.S. Chemistry
Truman State University
Kirksville, MO
USA
Stowers Microscopy Publications
Lange, J.J., Wood, C.J., Marshall, W.A., Maddera, L.E., Yu, Q.E., Bradford, W.D., Slaughter, B.D., Unruh, J.R. Wiegraebe, W. (2013). "Correction of bleaching artifacts in hih content fluorescence correlation spectroscopy (HCS-FCS) data". Proc. SPIE8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859006.
Other Publications

JJ Lange, CT Culbertson, DA Higgins (2008) “Single Molecule Studies of Solvent-Dependent Diffusion and Entrapment in Poly (dimethylsiloxane) Thin Films” Anal. Chem. 80 (24), 9726-9734.

KW Hoeman, JJ Lange, GT Roman, DA Higgins, CT Culbertson (2009) “Electrokinetic trapping using titania nanoporous membranes fabricated using sol–gel chemistry on microfluidic devices” Electrophoresis. 30 (18), 3160-3167.         

JJ Lange, MM Collinson, CT Culbertson, DA Higgins (2009) “Single-Molecule Studies of Oligomer Extraction and Uptake of Dyes in Poly (dimethylsiloxane) Films” Anal. Chem. 81 (24), 10089-10096.               

C Kong, D Samovski, P Srikanth, MJ Wainszelbaum, AJ Charron, J Liu, JJ Lange, P Chen, Z Pan, X Su, PD Stahl (2012) “Ubiquitination and degradation of the hominoid-specific oncoprotein TBC1D3 is mediated by CUL7 E3 ligase” PLOS ONE. 7 (9), e46485.         

C Kong, JJ Lange, D Samovski, X Su, J Liu, S Sundaresan, PD Stahl (2013) “Ubiquitination and degradation of the hominoid-specific oncoprotein TBC1D3 is regulated by protein palmitoylation” BBRC. 434 (2), 388-393.         

Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities

Web Links:
Carl Zeiss
Washington University
Kansas State University

Truman State University

Amanda Top
Lucinda

Members

Lucinda Maddera

Jeffrey Top
Sean Members
Lucinda Maddera

Lucinda Maddera
Research Technician III, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4331
Fax: (816) 926-4691
email: lem@stowers.org

2011 - present
 
2011 - present
Research Technician III, Stowers Microscopy Center
Stowers Institute for Medical Research
2010 - 2011
Senior Research Assistant
Oklahoma Medical Research Foundation
Oklahoma City, OK
USA
  M.S. Microbiology & Molecular Genetics
Oklahoma State University
Stillwater, OK
USA
Stowers Microscopy Publications
Lange, J.J., Wood, C.J., Marshall, W.A., Maddera, L.E., Yu, Q.E., Bradford, W.D., Slaughter, B.D., Unruh, J.R. Wiegraebe, W. (2013). "Correction of bleaching artifacts in hih content fluorescence correlation spectroscopy (HCS-FCS) data". Proc. SPIE8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859006.
Other Publications

Clarke, M. and Maddera, L. (2004).  Distribution of alkaline phosphatase in vegetative Dictyostelium cells in relation to the contractile vacuole complex.  Eur. J. Cell Biol. 83, 289-296.

Daehnel, K., Harrir, R., Maddera, L., and Silverman, P. (2005). Fluorescence assays for F-pili and their application. Micro. 151 (Pt 11), 3541-8.

Clarke, M., and Maddera, L. (2006).  Phagocyte meets prey: Uptake, internalization, and killing of bacteria by Dictyostelium amoebae.  Eur. J. Cell Biol. 85, 1001-1010.

Clarke, M., Maddera, L., Harris, R.L., and Silverman, P.M. (2008).  F-pili dynamics by live cell imaging.  Proc. Nat. Acad. Sci. USA 105, 17978-17981.

Clarke, M., Maddera, L., Engel, U., Gerisch, G. (2010). Retrieval of the vacuolar H+-ATPase from phagosomes revealed by live cell imaging. PLoS ONE 5, e8585.

Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities

Web Links:
Oklahoma Medical Research Foundation
Oklahoma State University

Jeffrey Top
Larger Image / Down Sean Members

Sean McKinney

Lucinda Top
Rick Members
Sean McKinney

Sean McKinney
Group Head, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4364
Fax: (816) 926-4691
email: smc@stowers.org

2009 - present
 
2011 - present
Group Head , Stowers Microscopy Center
Stowers Institute for Medical Research
2009 - 2011
Research Specialist II, Stowers Microscopy Center
Stowers Institute for Medical Research
2008 - 2009
Research Specialist
Janelia Farm Research Campus
Ashburn, VA
USA
2006 - 2008
Postdoctoral Research Fellow
Janelia Farm Research Campus
Ashburn, VA
USA
2001 - 2006
Graduate Research Fellow
University of Illinois Urbana-Champaign
Urbana, IL
USA
2006
Ph.D. Physics
University of Illinois Urbana-Champaign
Urbana, IL
USA
2001
B.S. Physics and Computer Science
University of Missouri Rolla
Rolla, MO
USA
Stowers Microscopy Publications
 

Adler CE, Seidel CW, McKinney SA, Sánchez alvarado A. Selective amputation of the pharynx identifies a FoxA-dependent regeneration program in planaria. Elife. 2014;3:e02238.

Nakajima Y, Meyer EJ, Kroesen A, McKinney SA, Gibson MC. Epithelial junctions maintain tissue architecture by directing planar spindle orientation. Nature. 2013;500(7462):359-62.

Xu N, Zhang SO, Cole RA, McKinney SA, Guo F, Haas JT, Bobba S, Farese RV Jr, Mak HY. The FATP1-DGAT2 complex facilitates lipid droplet expansion at the ER-lipid droplet interface. J Cell Biol. 2012;198(5):895-911.

Other Publications

Tian L, Hires SA, Mao T, Huber D, Chiappe ME, Chalasani SH, Petreaunu L, Akerboom J, McKinney SA, Schreiter ER, Bargmann CI, Jayaraman V, Svoboda K, Looger LL. Imaging neural activity in worms, flies and mice with improved GCaMP calcium indicators. Nat Methods. 2009;6(12):875-81.

McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. A bright and photostable photoconvertible fluorescent protein. Nat Methods. 2009;6(2):131-3.

Rasnik I, Jeong YJ, McKinney SA, Rajagopal V, Patel SS, Ha T. Branch migration enzyme as a Brownian ratchet. EMBO J. 2008;27(12):1727-35.

Rasnik I, McKinney SA, Ha T. Nonblinking and long-lasting single-molecule fluorescence imaging. Nat Methods. 2006;3(11):891-3.

Joo C, Mckinney SA, Nakamura M, Rasnik I, Myong S, Ha T. Real-time observation of RecA filament dynamics with single monomer resolution. Cell. 2006;126(3):515-27.

Mckinney SA, Joo C, Ha T. Analysis of single-molecule FRET trajectories using hidden Markov modeling. Biophys J. 2006;91(5):1941-51.

Toprak E, Enderlein J, Syed S, McKinney SA, Petschek RG, Ha T, Goldman YE, Selvin PR. Defocused orientation and position imaging (DOPI) of myosin V. Proc Natl Acad Sci USA. 2006;103(17):6495-9.

Buranachai C, McKinney SA, Ha T. Single molecule nanometronome. Nano Lett. 2006;6(3):496-500.

Rasnik I, McKinney SA, Ha T. Surfaces and orientations: much to FRET about?. Acc Chem Res. 2005;38(7):542-8.

McKinney SA, Freeman AD, Lilley DM, Ha T. Observing spontaneous branch migration of Holliday junctions one step at a time. Proc Natl Acad Sci USA. 2005;102(16):5715-20.

Liu J, Déclais AC, McKinney SA, Ha T, Norman DG, Lilley DM. Stereospecific effects determine the structure of a four-way DNA junction. Chem Biol. 2005;12(2):217-28.

Joo C, McKinney SA, Lilley DM, Ha T. Exploring rare conformational species and ionic effects in DNA Holliday junctions using single-molecule spectroscopy. J Mol Biol. 2004;341(3):739-51.

Yildiz A, Forkey JN, McKinney SA, Ha T, Goldman YE, Selvin PR. Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science. 2003;300(5628):2061-5.

McKinney SA, Déclais AC, Lilley DM, Ha T. Structural dynamics of individual Holliday junctions. Nat Struct Biol. 2003;10(2):93-7.

Stowers Links:
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Stowers Institute Core Facilities

Web Links:
Janelia Farm Research Campus
University of Illinois
Missouri University of Science and Technology

 

Cindy Top
Rick Members

Richard Pierce

Sean Top
Carolyn Members
Rick

Richard Pierce
Laboratory Technician I, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4460
Fax: (816) 926-4698
email:rap@stowers.org

2014- present

Laboratory Technician I, Stowers Microscopy Center
Stowers Institute for Medical Research

   

Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities

Web Links:
University of Missouri - Kansas City

Sean Top
Carolyn Members

Carolyn Randolph

Richard Top
Sarah Members

Carolyn Randolph
Senior Administrative Assistant, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4063
Fax: (816) 926-2027
email:cgr@stowers.org

2009 - present

Senior Administrative Assistant, Stowers Microscopy Center
Stowers Institute for Medical Research

   

Stowers Links:
Stowers Institute Home Page
Stowers Institute Core Facilities

Richard Top
Carolyn Members

Sarah Smith

Carolyn Top

Chris

Members

Sarah Smith
Research Specialist I, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4331
Fax: (816) 926-4691
email: sas@stowers.org

2013 - present

2007-2013

Ph.D., University of Kansas Medical Center
University of Kansas Medical Center

2005-2007

Senior Research Technician, Stowers Microscopy Center
Stowers Institute for Medical Research

2007 - 2013
Postdoctoral Research Associate, Rong LiLab
Stowers Institute for Medical Research
2005 - 2007
B.S. Physics, Minor Biology
Graduate Student
Truman State University
Kirskville, MO
Stowers Microscopy Publications

Smith SE, Slaughter BD, and Unruh J: Imaging methodologies for systems biology: Investigations of cell polarity.  Cell Adhesion & Migration 2014, Vol 8, Issue 4.

Smith SE, Rubinstein B, Mendes Pinto I, Slaughter BD, and Li R: Independence of symmetry breaking on Bem1-mediated autocatalytic activation of Cdc42.  J Cell Biol. 2013 Sep 30;202(7):1091-106.

Slaughter BD, Unruh J, Das A, Smith SE, Rubenstein B, and Li R:  Non-Uniform Membrane Diffusion Enables Steady-State Cell Polarization via Vesicular Trafficking.  Nature Communications 2013, Vol 4, Article 1380.

Slaughter BD, Smith SE and Li R: Symmetry breaking in the life cycle of the budding yeast. Cold Spring Harbor Perspect Biol 2009, 1(3):a003384.

Byers MA, Calloway PA, Shannon L, Cunningham HD, Smith SE, Li F, Fassold BC, and Vines CM: Arrestin 3 Mediates Endocytosis of CCR7 following Ligation of CCL19 but Not CCL21. J Immunol 2008, 181:4723-4732.

Jin Z, Kirilly D, Weng C, Kawase E, Song X, Smith SE, Schwartz J, and Xie T: Differentiation-Defective Stem Cells Outcompete Normal Stem Cells for Niche Occupancy in the Drosophila Ovary. Cell Stem Cell 2008, 2:39-49.

Kulesa PM, Teddy JM, Stark DA, Smith SE, and McLennan R: Neural crest invasion is a spatially-ordered progression into the head with higher cell proliferation at the migratory front as revealed by the photoactivatable protein, KikGR. Dev Biol 2008, 316:275-287.

Stowers Links:
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Web Links:
Truman State University
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Members

Christopher J. Wood

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Zulin Members
Christopher Wood Christopher J. Wood
Group Head, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4409
Fax: (816) 926-4691
email: cjw@stowers.org

2009 - present
 
2011 - present
Group Head, Stowers Microscopy Center
Stowers Institute for Medical Research
2009 - 2011
Senior Research Specialist, Stowers Microscopy Center
Stowers Institute for Medical Research
2006 - 2009

Research Specialist II, Stowers Microscopy Center
Stowers Institute for Medical Research

2003 - 2006

Senior Project Engineer
Engineering Methods, Inc.
Cincinnati, OH
USA

2000 - 2003

Software Development/Network Administration
Imagination Engineering
Kearney, MO
USA

1998 - 2000

Project Engineer
ITD Associates
Liberty, MO
USA

1995 - 1998

Research/Teaching Assistant
University of Missouri-Rolla
Rolla, MO
USA

1993 - 1998
PhD - Physics: "Dynamics of double electron removal from helium and molecular hydrogen by heavy ions"
University of Missouri-Rolla
Rolla, MO
USA
Stowers Microscopy Publications

Lange, J.J., Wood, C.J., Marshall, W.A., Maddera, L.E., Yu, Q.E., Bradford, W.D., Slaughter, B.D., Unruh, J.R. Wiegraebe, W. (2013). "Correction of bleaching artifacts in hih content fluorescence correlation spectroscopy (HCS-FCS) data". Proc. SPIE8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859006.

Stowers Links:
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Stowers Institute Core Facilities

Web Links:
Engineering Methods, Inc.
University of Missouri-Rolla
Iowa State University

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Members

Zulin Yu

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Michael Members
Christopher Wood

Zulin Yu
Research Specialist II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

Phone: (816) 926-4303
Fax: (816) 926-4691
email :zyu@stowers.org

2010 - present
 
2008 - 2010
Postdoctoral Research Associate, Ron Yu Lab
Stowers Institute for Medical Research
2003 - 2008

Ph.D. Biophysics, Institute of Biophysics, Beijing, China
Institute of Biophysics, Chinese Academy of Sciences

1999-2003
B.S., Biophysics, Nankai University, Tianjin, China
Nankai University
Stowers Microscopy Publications

Collins KA, Unruh JR, Slaughter BD, Yu Z, Lake CM, et al. (2014) Corolla Is a Novel Protein that Contributes to the Architecture of the Synaptonemal Complex of Drosophila. Genetics: genetics. 114.165290.

Other Publications

Shen J-X, Xu Z-M, Yu Z-L, Wang S, Zheng D-Z, et al. (2011) Ultrasonic frogs show extraordinary sex differences in auditory frequency sensitivity. Nature communications 2: 342.

Wu J, Yu Z, Mai X, Wei J, Luo Y (2011) Pitch and loudness information encoded in auditory imagery as revealed by event‐related potentials. Psychophysiology 48: 415-419.

Wu J, Mai X, Yu Z, Qin S, Luo YJ (2010) Effects of discrepancy between imagined and perceived sounds on the N2 component of the event‐related potential. Psychophysiology 47: 289-298.

Feng AS, Riede T, Arch VS, Yu Z, Xu ZM, et al. (2009) Diversity of the Vocal Signals of Concave‐Eared Torrent Frogs (Odorrana tormota): Evidence for Individual Signatures. Ethology 115: 1015-1028.

Feng AS, Arch VS, Yu Z, Yu XJ, Xu ZM, et al. (2009) Neighbor–Stranger Discrimination in Concave‐Eared Torrent Frogs, Odorrana tormota. Ethology 115: 851-856.

Shen J-X, Feng AS, Xu Z-M, Yu Z-L, Arch VS, et al. (2008) Ultrasonic frogs show hyperacute phonotaxis to female courtship calls. Nature 453: 914-916.

Gridi-Papp M, Feng AS, Shen J-X, Yu Z-L, Rosowski JJ, et al. (2008) Active control of ultrasonic hearing in frogs. Proceedings of the National Academy of Sciences 105: 11014-11019.

Qiu Q, Tang J, Yu Z, Zhang J, Zhou Y, et al. (2007) Latency represents sound frequency in mouse IC. Science in China Series C: Life Sciences 50: 258-264.

Feng AS, Narins PM, Xu C-H, Lin W-Y, Yu Z-L, et al. (2006) Ultrasonic communication in frogs. Nature 440: 333-336.

Yu Z-L, Qiu Q, Xu Z-M, Shen J-X (2006) Auditory response characteristics of the piebald odorous frog and their implications. Journal of Comparative Physiology A 192: 801-806.

Stowers Links:
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Alumni - Michael Adams

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Michael Members
MAD

Michael Adams
Research Technician II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

 

2009 - 2011
Research Technician II, Stowers Microscopy Center
Stowers Institute for Medical Research
Medical Physicist Intern
University of Kansas Medical Center-Radiation Oncology
Kansas City, KS USA
MS. Physics
University of Missouri-Kansas City
Kansas City, MO
USA

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Web Links:
University of Missouri - Kansas City
University of Kansas Medical Center-Radiation Oncology

 

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Dave

Members

Alumni - Shiqiang Dai

Michael Top
John Members
Shiqiang Dai

Shiqiang Dai
Research Technician II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

 

2009 - 2013
Research Technician II, Stowers Microscopy Center
Stowers Institute for Medical Research
2008 - 2009
Joint Graduate Student, Stowers Microscopy Center
Stowers Institute for Medical Research

University of Missouri - Kansas City

2007 - 2009
2004 - 2006
Research Assistant
North Dakota State University
 

M.S. -Electrical Engineering
North Dakota State University
Fargo, ND
USA

  B.S. Electrical Engineering
CHANGCHUN University of Technology
ChangChun City
China

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Web Links:
University of Missouri - Kansas City
North Dakota State University
CHANGCHUN University of Technology

 

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Alumni - John Dirnberger

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Joseph Members
John Dirnberger John Dirnberger
Research Technician II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110
2008 - 2010
Research Technician II, Stowers Microscopy Center
Stowers Institute for Medical Research
 

B.S. Biology
Truman State University
Kirksville, MO
USA

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Truman State University

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Joseph Members

Alumni - Joseph Huff

John Top
Kasthuri Members
Joseph Huff

Joseph Huff
Research Technician II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

2005 - 2008
Research Technician II, Advanced Instrumentation & Physics
Stowers Institute for Medical Research
  M.S. Physics
Creighton University
Omaha, NE
USA
  B.A. Physics
William Jewell College
Liberty, MO
USA

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Web Links:
Creighton University
William Jewell College

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Alumni - Kasthuri Kannan

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Dmitri Members

 

Kasthuri Kannan
Research Specialist II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

2008 - 2010
Research Specialist II, Stowers Microscopy Center
Stowers Institute for Medical Research
 

Ph.D. - Computer Science
M.S. -Mathematics
Texas A&M University
College Station, TX
USA

  M.S. - Mathematics
Indian Institute of Technology, Madras
Madras
India
  B.S. - Mathematics
University of Madras
Madras
India

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Web Links:
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Texas A&M University
Indian Institute of Technology, Madras
University of Madras

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Alumni - Dmitri Lastochkin

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Katie Members

Dmitri Lastochkin

Dmitri Lastochkin
Research Specialist II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

2008 - 2008
Research Specialist II, Advanced Instrumentation and Physics
Stowers Institute for Medical Research
Research Scientist
University of Notre Dame
Research Scientist
University of Houston
Senior Researcher
Technion
Teaching Assistant
Technion

Leading Engineer
Aircraft Technology Research Center

Research Assistant
Optical and Physical Measurements Research Institute
 

Ph.D. - Chemical Engineering
Technion
Haifa
Israel

 

Ph.D.. - Physical and Quantum Electronics
Optical and Physical Measurements Research Institute
M.Sc. - Physics
Moscow State Engineering Physics Institute
Moscow
Russia

Stowers Links:
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Web Links:
Univerisity of Notre Dame
University of Houston
Technion
Israel Plastic and Rubber Center
National Research Centre Kurchatov Institute
Moscow State Engineering Physics Institute

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Alumni - William A. Marshall

Dmitri

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Katie Members
Will Marshall

Will Marshall
Research Technician II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

 

2008 - 2012
Research Technician II, Stowers Microscopy Center
Stowers Institute for Medical Research
2007 - 2008
Medical Research Lab Technician
University of Missouri-Columbia
Columbia, MO
USA
  B.S. Biochemistry
University of Missouri-Columbia
Columbia, MO
USA

Stowers Links:
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Web Links:
University of Missouri-Columbia

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Alumni - Katherine Perko Hollander

Will Top
Joel Members
Katherine Perko

Katherine Perko Hollander
Research Technician II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

2007 - 2010
Research Technician II, Stowers Microscopy Center
Stowers Institute for Medical Research
  B.A. Chemistry
William Jewell College
Liberty, MO
USA

Stowers Links:
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Web Links:
William Jewell College

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Alumni - Mark Richardson

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Joel Members
Mark Richardson
Senior Microscopy Technician, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

2011 - 2014
Senior Microscopy Technician, Stowers Microscopy Center
Stowers Institute for Medical Research
1997-2011
Sales Consultant, Carl Zeiss Microimaging, LLC
Carl Zeiss Microimaging LLC

Stowers Links:
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Web Links:
Carl Zeiss

Mark Members
Members

Alumni - Joel Schwartz

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Miranda Members
Joel Schwartz Joel Schwartz
Managing Director of Imaging, Imaging Center
1000 East 50th Street
Kansas City, MO 64110
2004 - 2008
Managing Director of Imaging, Imaging Center
Stowers Institute for Medical Research
2008
Adjunct Assistant Professor, Department of Chemistry
University of Kansas
Lawrence, KS
USA
Postdoctoral Research Fellow
Vanderbilt University
Nashville, TN
USA
  PhD - Neuroscience
Vanderbilt University
Nashville, TN
USA
  B.S./B.A. Biochemistry/Chemistry
Virginia Polytechnic Institute and State University
Blascksburg, VA
USA

Stowers Links:
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Web Links:
University of Kansas
Vanderbilt University
Virginia Polytechnic Institute and State University

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Alumni - Miranda Smith

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Jay Members
Miranda Smith

Miranda Smith
Research Technician II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

2006 - 2009
Research Technician II, Stowers Microscopy Center
Stowers Institute for Medical Research
  B.S. Cellular/Molecular Biology
Northwest Missouri State University
Maryville, MO
USA

Stowers Links:
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Web Links:
Northwest Missouri State University

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Alumni - Jay Unruh

Miranda Top
Elden Members
Jay Unruh

Jay Unruh
Research Specialist II, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

2007 - 2010
Research Specialist II, Stowers Microscopy Center
Stowers Institute for Medical Research
2006 - 2007
  Ph.D. Chemistry, "Development of Fluorescence Spectroscopy Tools for the Measurement of Biomolecular Dynamics and Heterogeneity"
University of Kansas
Lawrence, KS
USA
  B.S. Biochemistry
John Brown University
Siloam Springs, AR
USA

Stowers Links:
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Web Links:
University of California-Irvine
Laboratory for Fluorescence Dynamics
University of Kansas
Carey Johnson Group
John Brown University

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\

Alumni - Qingfeng Yu

Jay Top
Members
Christopher Wood Qingfeng 'Elden' Yu
Research Specialist I, Stowers Microscopy Center
1000 East 50th Street
Kansas City, MO 64110

2010 - 2014
Research Specialist I, Stowers Microscopy Center
Stowers Institute for Medical Research
2010
Research Specialist, Ron Yu Lab
Stowers Institute for Medical Research
2005
Associate Statistical Analyst Intern
Hewlett-Packard
Austin, TX
USA
2004
Visiting Student
Microsoft Research Asia
Beijing
China
2003
Graduate Intern
Hewlett-Packard
Austin, TX
USA
Stowers Microscopy Publications
 

Lange, J.J., Wood, C.J., Marshall, W.A., Maddera, L.E., Yu, Q.E., Bradford, W.D., Slaughter, B.D., Unruh, J.R. Wiegraebe, W. (2013). "Correction of bleaching artifacts in hih content fluorescence correlation spectroscopy (HCS-FCS) data". Proc. SPIE8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859006.

Ma, L., Qiu, Q., Gradwohl, S., Scott, A., Yu, E.Q., Alexander, R., Wiegraebe, W. (2012). "Distributed representation of chemical and tunotopic organization of glomeruli in the mouse olfactory bulb". PNAS. 109(14): 5481-5486.

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Web Links:
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Hewlett-Packard
Microsoft Research Asia

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For comments and additional information, please contact Winfried Wiegraebe. Red links are for internal use only. Sorry!