The Stowers Institute For Medical Research: Microscopy Center

Stowers
Microscopy Center
Director: Winfried Wiegraebe
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Simulate Dual Color FCS and PCH Data

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Dual Color FCS Simulator

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This applet will allow you to simulate FCS data with molecules of different brightnesses in two detection channels. You can then analyze the data using standard FCS and PCH fitting methods.

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FCS Simulator

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Details about the Simulation

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The simulation takes place in a 3.2 µm box. The beam radial waist is 0.17 µm with an axial dimension of 0.7 µm. The number of frames will always be truncated to a power of 2 for expediated processing. The other parameters are, for the most part, self-explanatory. Note that java applets are by nature limited because of memory restrictions. For more thorough simulations and analysis see the software link to the right.

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PCH Analysis Software
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For comments and additional information, please contact Winfried Wiegraebe. Red links are for internal use only. Sorry!