The Stowers Institute For Medical Research: Microscopy Center

Stowers
Microscopy Center
Head: Winfried Wiegraebe

OverviewTechnologyInstrumentationImage ProcessingProjectsSeminarsPeopleLinksSite Map

Overview
Technology
Microscopy
Two-Photon
Fluorescence Correlation Spectroscopy
Instrumentation
Help
Reservation
Microscope Training
Microscope Maintenance
Microscope Cleaning
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Image Processing
Software
Projects
Art and Neuroscience
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Site Map

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Overview

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Stowers Microscopy Center

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Light is one of the most powerful tools to probe biology. We make it available to research laboratories at Stowers Institute. Our focus is on high resolution light microscopy, confocal microscopy (LSM) , non-linear optics (NLO) and fluorescence correlation spectroscopy (FCS) . You can find our internal pages at Stowers Microscopy Center.

Stowers Links:
Research Faculty
Stowers Microscopy Center
Stowers Microscopy Center: Microscopy
Stowers Microscopy Center: Technology
Stowers Microscopy Center: Two-Photon Microscopy
Stowers Microscopy Center: FCS
Stowers Microscopy Center internal

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Technology

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All technologies we are currently using are based on light microscopy. To get three-dimensional information we use confocal Laser Scanning Microscopes (LSM). To image thicker, highly scattering and sensitive live samples we have combined some of these systems with two photon excitation. Fluorescence Correlation Spectroscopy (FCS) allows us to probe molecular diffusion and interaction in solution and living cells.

In most cases we rely on fluorescence as contrast mechanism, but we are working on Fluorescence Lifetime Imaging (FLIM) and Second Harmonic Generation (SHG) as well.

For data evaluation we use standard software packages. For special applications we use IDL and Mathematica.

For details, please visit our Technology pages:

Stowers Links:
Stowers Microscopy Center: Technology
Stowers Microscopy Center: Microscopy
Stowers Microscopy Center: Two-Photon Microscopy, Non-Linear Optics (NLO)
Stowers Microscopy Center: FCS
Stowers Microscopy Center: Software
Stowers Microscopy Center: Instruments

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Projects

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All of our projects are in close collaboration with laboratories at the Stowers Institute. This page describes some of the topics we are working on, including transcription involving RNA-Polymerase II, imaging of the fly brain and Second Harmonic Generation (SHG) to measure membrane potentials. These pages are mainly intended for internal communication, but feel free to browse them.

For details, please visit our Projects page:

Stowers Links:
Stowers Microscopy Center: Projects
Research Faculty

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Seminars

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Technology & Methods

On the last Thursday of every month, we organize a Technology and Methods Seminar. We present technologies offered at the Stowers Institute and their applications.

Foundations of Microscopy

On the 1st and 3rd Friday of the month we discuss fundamentals in microscopy and the related theoretical basics. These seminars are intended for people who want to understand the inner workings of a microscope in order to improve their scientific results based on optical imaging or modify and built microscopes and related optical set-ups.

Foundations of Image Processing

Image processing is becoming a major part of microscopy. On the 4th Friday of the month we discuss theory and implementation of image processing algorithms.The seminars are intended for users of image processing software who want to ensure optimal results while avoiding artifacts. We will discuss different software implementations and how to develop new algorithms.

FCS User Club

Once a month we are disussing classic papers about FCS and related techniques. The FCS User Club is a forum for current and potential users of fluorescence correlation spectroscopy.

For details, please visit our Seminars page:

Stowers Links:
Stowers Microscopy Center: Seminars
Stowers Bioplog

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People

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For details, please visit our People page.

Stowers Links:
Stowers Microscopy Center: People

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Links and Downloads

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We have collected links to references, companies and downloads related to microscopy and the projects we are working at. In addition you will find some information about Kansas City.

Please let us know, if you come across some web pages we should add.

For details, please visit our Links page:

Stowers Links:
Stowers Microscopy Center: Links

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Site Map for Stowers Microscopy Center and the Stowers Image Center

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A summary of all pages maintained by Stowers Microscopy Center and the Imaging Center can be found here. Please let us know if you have difficulties navigating this site or want us to add or remove some information.

Stowers Links:
Stowers Microscopy Center: Site Map

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For comments and additional information, please contact Winfried Wiegraebe. Red links are for internal use only. Sorry!