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Shilatifard Publications
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The JARID2-PRC2 duality. Herz HM, Shilatifard A. Genes Dev. 2010 May;24(9):857-61. The H3K27me3-demethylase dUTX is a suppressor of Notch- and Rb-dependent tumors in Drosophila. Herz HM, Madden LD, Chen Z, Bolduc C, Buff E, Gupta R, Davuluri R, Shilatifard A, Hariharan IK, Bergmann A. Mol Cell Biol. 2010 Mar 8. [Epub ahead of print] Linking H3K79 trimethylation to Wnt signaling through a novel Dot1-containing complex (DotCom). Mohan M, Herz HM, Takahashi YH, Lin C, Lai KC, Zhang Y, Washburn MP, Florens L, Shilatifard A. Genes Dev. 2010 Mar 15;24(6):574-89. Epub 2010 Mar 4. Lin C, Smith ER, Takahashi H, Lai KC, Martin-Brown S, Florens L, Washburn MP, Conaway JW, Conaway RC, Shilatifard A. Mol Cell. 2010 Feb 12;37(3):429-37. Structural basis for H3K4 trimethylation by yeast Set1/COMPASS. Takahashi YH, Shilatifard A. Adv Enzyme Regul. 2009 Dec 18. The Curious Case of Bivalent Marks. Herz HM, Nakanishi S, Shilatifard A. Developmental Cell 2009. [published ahead of print August 14, 2009]. Molecular Implementation and Physiological Roles for Histone H3 Lysine 4 (H3K4) Methylation. Shilatifard A. Curr Opin Cell Biol. Author manuscript; available in PMC 2009 June 1. RAD6-Mediated transcription-coupled H2B ubiquitylation directly stimulates H3K4 methylation in human cells. Kim J, Guermah M, McGinty RK, Lee JS, Tang Z, Milne TA, Shilatifard A, Muir TW, Roeder RG. Cell. 2009 May 1;137(3):459-71. Regulation of H3K4 trimethylation via Cps40 (Spp1) of COMPASS is monoubiquitination-independent: Implication for a Phe/Tyr switch by the catalytic domain of Set1. An operational definition of epigenetics. Berger SL, Kouzarides T, Shiekhattar R, Shilatifard A. Genes Dev. 2009 Apr 1;23(7):781-3. Histone H3 Lysine 36 Dimethylation (H3K36me2) Is Sufficient to Recruit the Rpd3s Histone Deacetylase Complex and to Repress Spurious Transcription. Li B, Jackson J, Simon MD, Fleharty B, Gogol M, Seidel C, Workman JL, Shilatifard A. J Biol Chem. 2009 Mar 20;284(12):7970-6. Epub 2009 Jan 20. Developmental biology. Histone cross-talk in stem cells. Smith E, Shilatifard A. Molecular regulation of H3K4 trimethylation by Wdr82, a component of the human Set1/COMPASS. Wu M, Wang PF, Lee JS, Martin-Brown S, Florens L, Washburn M, Shilatifard A. Mol Cell Biol. 2008 Oct 6. A comprehensive library of histone mutants identifies nucleosomal residues required for H3K4 methylation. Nakanishi S, Sanderson BW, Delventhal KM, Bradford WD, Staehling-Hampton K, Shilatifard A. Nat Struct Mol Biol. 2008 Aug;15(8):881-8. Epub 2008 Jul 11. Regulation of the transcriptional activity of poised RNA polymerase II by the elongation factor ELL. Smith ER, Winter B, Eissenberg JC, Shilatifard A. Molecular implementation and physiological roles for histone H3 lysine 4 (H3K4) methylation. Shilatifard, A. Curr Opin Cell Biol. 2008 May 26. Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice. Wang P, Bowl MR, Bender S, Peng J, Farber L, Chen J, Ali A, Zhang Z, Alberts AS, Thakker RV, Shilatifard A, Williams BO, Teh BT.
Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8. Henry KW, Wyce A, Lo WS, Duggan LJ, Emre NC, Kao CF, Pillus L, Shilatifard A, Osley MA, Berger SL. Genes Dev. 2003 Nov 1;17(21):2648-63. Epub 2003 Oct 16.
The Paf1 complex is essential for histone monoubiquitination by the Rad6-Bre1 complex, which signals for histone methylation by COMPASS and Dot1p. Wood A, Schneider J, Dover J, Johnston M, Shilatifard A. J Biol Chem. 2003 Sep 12;278(37):34739-42. Epub 2003 Jul 21. Molecular basis of p53 functional inactivation by the leukemic protein MLL-ELL. Wiederschain D, Kawai H, Gu J, Shilatifard A, Yuan ZM.
Methylation of histone H3 by Set2 in Saccharomyces cerevisiae is linked to transcriptional elongation by RNA polymerase II. Krogan NJ, Kim M, Tong A, Golshani A, Cagney G, Canadien V, Richards DP, Beattie BK, Emili A, Boone C, Shilatifard A, Buratowski S, Greenblatt J. Mol Cell Biol. 2003 Jun;23(12):4207-18.
Transcriptional elongation by RNA polymerase II and histone methylation. Gerber M, Shilatifard A. J Biol Chem. 2003 Jul 18;278(29):26303-6. Epub 2003 May 22. Review. The RNA polymerase II elongation complex. Shilatifard A, Conaway RC, Conaway JW. The Paf1 complex is required for histone H3 methylation by COMPASS and Dot1p: linking transcriptional elongation to histone methylation. Krogan NJ, Dover J, Wood A, Schneider J, Heidt J, Boateng MA, Dean K, Ryan OW, Golshani A, Johnston M, Greenblatt JF, Shilatifard A. Mol Cell. 2003 Mar;11(3):721-9.
Bre1, an E3 ubiquitin ligase required for recruitment and substrate selection of Rad6 at a promoter. Wood A, Krogan NJ, Dover J, Schneider J, Heidt J, Boateng MA, Dean K, Golshani A, Zhang Y, Greenblatt JF, Johnston M, Shilatifard A. Mol Cell. 2003 Jan;11(1):267-74.
RNA polymerase II elongation factors of Saccharomyces cerevisiae: a targeted proteomics approach. Krogan NJ, Kim M, Ahn SH, Zhong G, Kobor MS, Cagney G, Emili A, Shilatifard A, Buratowski S, Greenblatt JF. Mol Cell Biol. 2002 Oct;22(20):6979-92.
dELL is an essential RNA polymerase II elongation factor with a general role in development. Eissenberg JC, Ma J, Gerber MA, Christensen A, Kennison JA, Shilatifard A. Methylation of histone H3 by COMPASS requires ubiquitination of histone H2B by Rad6. Dover J, Schneider J, Tawiah-Boateng MA, Wood A, Dean K, Johnston M, Shilatifard A. COMPASS, a histone H3 (Lysine 4) methyltransferase required for telomeric silencing of gene expression. Krogan NJ, Dover J, Khorrami S, Greenblatt JF, Schneider J, Johnston M, Shilatifard A. J Biol Chem. 2002 Mar 29;277(13):10753-5. Epub 2002 Jan 22.
Drosophila ELL is associated with actively elongating RNA polymerase II on transcriptionally active sites in vivo. Gerber M, Ma J, Dean K, Eissenberg JC, Shilatifard A. COMPASS: a complex of proteins associated with a trithorax-related SET domain protein. Miller T, Krogan NJ, Dover J, Erdjument-Bromage H, Tempst P, Johnston M, Greenblatt JF, Shilatifard A. Proc Natl Acad Sci U S A. 2001 Nov 6;98(23):12902-7. Epub 2001 Oct 30. Erratum in: Proc Natl Acad Sci U S A 2001 Dec 18;98(26):15393-4.
Muf1, a novel Elongin BC-interacting leucine-rich repeat protein that can assemble with Cul5 and Rbx1 to reconstitute a ubiquitin ligase. Kamura T, Burian D, Yan Q, Schmidt SL, Lane WS, Querido E, Branton PE, Shilatifard A, Conaway RC, Conaway JW. J Biol Chem. 2001 Aug 10;276(32):29748-53. Epub 2001 May 30.
Cloning and characterization of ELL-associated proteins EAP45 and EAP20. a role for yeast EAP-like proteins in regulation of gene expression by glucose. Kamura T, Burian D, Khalili H, Schmidt SL, Sato S, Liu WJ, Conrad MN, Conaway RC, Conaway JW, Shilatifard A. Transcription factors TFIIF, ELL, and Elongin negatively regulate SII-induced nascent transcript cleavage by non-arrested RNA polymerase II elongation intermediates. Elmendorf BJ, Shilatifard A, Yan Q, Conaway JW, Conaway RC. J Biol Chem. 2001 Jun 22;276(25):23109-14. Epub 2001 Mar 19. Functional analysis of the leukemia protein ELL: evidence for a role in the regulation of cell growth and survival. Johnstone RW, Gerber M, Landewe T, Tollefson A, Wold WS, Shilatifard A. Mol Cell Biol. 2001 Mar;21(5):1672-81. A carboxy-terminal domain of ELL is required and sufficient for immortalization of myeloid progenitors by MLL-ELL. DiMartino JF, Miller T, Ayton PM, Landewe T, Hess JL, Cleary ML, Shilatifard A. Blood. 2000 Dec 1;96(12):3887-93. Control of elongation by RNA polymerase II. Conaway JW, Shilatifard A, Dvir A, Conaway RC Identification, cloning, expression, and biochemical characterization of the testis-specific RNA polymerase II elongation factor ELL3. Miller T, Williams K, Johnstone RW, Shilatifard A. J Biol Chem. 2000 Oct 13;275(41):32052-6.
Human PC4 is a substrate-specific inhibitor of RNA polymerase II phosphorylation. Schang LM, Hwang GJ, Dynlacht BD, Speicher DW, Bantly A, Schaffer PA, Shilatifard A, Ge H, Shiekhattar R. J Biol Chem. 2000 Mar 3;275(9):6071-4.
The amino terminus of the mixed lineage leukemia protein (MLL) promotes cell cycle arrest and monocytic differentiation. Caslini C, Shilatifard A, Yang L, Hess JL. Cloning and characterization of the EAP30 subunit of the ELL complex that confers derepression of transcription by RNA polymerase II. Schmidt AE, Miller T, Schmidt SL, Shiekhattar R, Shilatifard A. J Biol Chem. 1999 Jul 30;274(31):21981-5. The elongin B ubiquitin homology domain. Identification of Elongin B sequences important for interaction with Elongin C. Brower CS, Shilatifard A, Mather T, Kamura T, Takagi Y, Haque D, Treharne A, Foundling SI, Conaway JW, Conaway RC. J Biol Chem. 1999 May 7;274(19):13629-36.
Inhibition of transcription by the trimeric cyclin-dependent kinase 7 complex. Bochar DA, Pan ZQ, Knights R, Fisher RP, Shilatifard A, Shiekhattar R. Factors regulating the transcriptional elongation activity of RNA polymerase II. Shilatifard A. Identification and purification of the Holo-ELL complex. Evidence for the presence of ELL-associated proteins that suppress the transcriptional inhibitory activity of ELL. Shilatifard A. J Biol Chem. 1998 May 1;273(18):11212-7.
The RNA polymerase II general elongation complex. Shilatifard A. Biol Chem. 1998 Jan;379(1):27-31. Review.
Structure and function of RNA polymerase II elongation factor ELL. Identification of two overlapping ELL functional domains that govern its interaction with polymerase and the ternary elongation complex. Shilatifard A, Haque D, Conaway RC, Conaway JW. J Biol Chem. 1997 Aug 29;272(35):22355-63.
ELL2, a new member of an ELL family of RNA polymerase II elongation factors. Shilatifard A, Duan DR, Haque D, Florence C, Schubach WH, Conaway JW, Conaway RC. Mechanism and regulation of transcriptional elongation and termination by RNA polymerase II. Shilatifard A, Conaway JW, Conaway RC. Curr Opin Genet Dev. 1997 Apr;7(2):199-204.
Expression of human H-type alpha1,2-fucosyltransferase encoding for blood group H(O) antigen in Chinese hamster ovary cells. Evidence for preferential fucosylation and truncation of polylactosamine sequences. Prieto PA, Larsen RD, Cho M, Rivera HN, Shilatifard A, Lowe JB, Cummings RD, Smith DF. J Biol Chem. 1997 Jan 24;272(4):2089-97.
Transcription syndromes and the role of RNA polymerase II general transcription factors in human disease. Aso T, Shilatifard A, Conaway JW, Conaway RC. An RNA polymerase II elongation factor encoded by the human ELL gene. Shilatifard A, Lane WS, Jackson KW, Conaway RC, Conaway JW. Science. 1996 Mar 29;271(5257):1873-6.
Sulphate release from keratan sulphate and heparan sulphate by bovine N-acetylglucosamine-6-sulphate sulphatase. Shilatifard A, Cummings RD. Purification and characterization of N-acetylglucosamine-6-sulfate sulfatase from bovine kidney: evidence for the presence of a novel endosulfatase activity. Shilatifard A, Cummings RD. Biochemistry. 1994 Apr 12;33(14):4273-82.
Complex-type N-linked oligosaccharides of gp120 from human immunodeficiency virus type 1 contain sulfated N-acetylglucosamine. Shilatifard A, Merkle RK, Helland DE, Welles JL, Haseltine WA, Cummings RD. J Virol. 1993 Feb;67(2):943-52.
gp160 of HIV-I synthesized by persistently infected Molt-3 cells is terminally glycosylated: evidence that cleavage of gp160 occurs subsequent to oligosaccharide processing. Merkle RK, Helland DE, Welles JL, Shilatifard A, Haseltine WA, Cummings RD
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