Notes

Slide Show

Outline

1	Basics in Microscopy: Transmitted Light
2	Magnification
3	Resolution
4	Contrast
5	The Eye and The Thin Lens
6	Simple Lens Produces Curved Image
7	To Get Things Larger Bring Them Closer
8	To Get Things Larger Bring Them Closer
9	… And Change Focus
10	… And Change Focus
11	Magnification
12	… And Add Magnifier Glas
13	… And Add Magnifier Glas
14	Microscope (Infinity Optics)
15	Magnification (Infinity Optics)
16	Tube Lens and Magnification
17	Calibration of Magnification
18	Newton and the Prism
19	Correction of Infinity Optics is Manufacturer Dependent
20	Abbe and the Resolution Limit
21	Airy Disk and Resolution
22	Light as Electromagnetic Wave
23	Plane Wave Focused by Lens
24	Constructive Interference
25	Destructive Interference
26	Diffraction Through Aperture
27	Diffracted Light
28	Two Apertures
29	Diffraction: Change of Spacing of Details
30	Diffraction: Change of Wavelength
31	Collect at Least First Order
32	Numerical Aperture
33	Add Immersion Oil
34	Add Immersion Oil
35	Add Immersion Oil
36	Use High N.A. Condenser
37	Illumination
38	The Lamp House
39	Condenser and Illumination
40	Köhler and Illumination
41	Köhler Illumination Even illumination Focus Condensor No stray light Adjust Field Stop Optimize resolution vs. contrast (depth of field) Adjust Aperture
42	Image Forming Beam Path
43	Image Forming Beam Path
44	Köhler Illumination: Focus Field Stop
45	Köhler Illumination: Center Field Stop
46	Pupil Beam Path
47	Köhler Illumination: Adjust Aperture
48	Köhler Illumination: Adjust Aperture
49	Image and Pupil Path are Interleaved
50	Fine Structures Enhanced in Front of Dark Background
51	Darkfield: Detect Diffracted Light Only
52	Darkfield: Detect Diffracted Light Only
53	Darkfield: Detect Diffracted Light Only
54	Phase Contrast Probes Refractive Index
55	Light is Slowed Down
56	Cell Adds Phase Shift
57	Interference After Phase Shift
58	Phase Contrast is Based on Interference
59	Phase Contrast is Based on Interference
60	Phase Contrast is Based on Interference
61	DIC Probes Differences in Refractive Index
62	Two Beams Probe Cell
63	Two Beams Probe Cell
64	Two Beams Probe Cell
65	Polarizer Selects One Direction
66	Wollaston Prism Splits Polarized Light
67	DIC (Normaski Contrast)
68	Phase Shift Rotates Beam
69	Brochures available as PDF
70	Molecular Expressions Webpage