Publications
2024
C G composition in transposon-derived genes is increased in FXD with perturbed immune system.
Suganuma, T., Hassan, H., Gogol, M., and Workman, J.L. (2024).
NAR Molecular Medicine, 1, ugae015
An Integrated Structural Model of the DNA Damage Responsive H3K4me3 Binding WDR76:SPIN1 Complex with the Nucleosome
Liu X, Zhang Y, Wen Z, Hao Y, Banks CAS, Cesare J, Bhattacharya S, Arvindekar S, Lange JJ, Xie Y, Garcia BA, Slaughter BD, Unruh JR, Viswanath S, Florens L, Workman JL, Washburn MP.
Proc Natl Acad Sci U S A. 2024;121:e2318601121
Rpd3S meets the nucleosome
Carrozza MJ, Workman JL. Cell Res. 2024;34:1-2
2023
Chromatin balances cell redox and energy homeostasis Sugamuna T, Workman J. Epigenet Chromatin. 2023;16:46.
https://doi.org/10.1186/s13072-13023-00520-13078.
The Swi-Snf chromatin remodeling complex mediates gene repression through metabolic controlChurch MC, Price A, Li H, Workman JL.
(2023).
Nucleic Acids Res. :gkad711. doi: 10.1093/nar/gkad711..
Paraspeckles interact with SWI/SNF subunit ARID1B to regulatetranscription and splicingReddy, D., Bhattacharya, S., Levy, M., Zhang, Y., Gogol, M., Li, H.,Florens, L., and Workman, J.L.
(2023).
EMBO Rep 24, e55345.
Serial Capture Affinity Purification and Integrated Structural Modeling of the H3K4me3 Binding and DNA Damage Related WDR76:SPIN1 ComplexLiu, X., Zhang, Y., Wen, Z., Hao, Y., Banks, C.A.S., Lange, J.J.,Cesare, J., Bhattacharya, S., Slaughter, B.D., Unruh, J.R., Florens, L., Workman,J.L., and Washburn, M.P. (2023).
bioRxiv DOI: 10.1101/2023.01.31.526478..
(mis)-Targeting of SWI/SNF complex(es) in cancerReddy, D., Bhattacharya, S., and Workman, J.L. (2023)
Cancer Metastasis Rev DOI: 10.1007/s10555-023-10102-5.
2022
Elevated levels of the methyltransferase SETD2 causes transcription andalternative splicing changes resulting in oncogenic phenotypesBhattacharya, S., Reddy, D., Zhang, N., Li, H., and Workman, J.L.(2022).
Front Cell Dev Biol 10, 945668.
MPTAC links alkylation damage signaling to sterol biosynthesis Suganuma T, Workman JL.
Redox Biol. 2022;51:102270. doi: 102210.101016/j.redox.102022.102270.
Original Data
MOCS2 links nucleotide metabolism to nucleoli function Suganuma T, Swanson SK, Gogol M, Garrett TJ, Florens L, Workman JL.
J Mol Cell Biol. 2022;13:838-840.
Original Data
The linker histone Hho1 modulates the activity of ATP-dependent chromatin remodeling complexes. Amigo R, Farkas C, Gidi C, Hepp MI, Cartes N, Tarifeno E, Workman JL, Gutierrez JL. [published ahead of print December 25 2021].
Biochim Biophys Acta Gene Regul Mech. 2022;1865:194781.
2021
The SAGA core module is critical during Drosophila oogenesis and is broadly recruited to promoters Soffers JHM, Alcantara SG, Li X, Shao W, Seidel CW, Li H, Zeitlinger J, Abmayr SM, Workman JL.
PLoS Genet. 2021;17:e1009668. doi: 10.1371/journal.pgen.1009668.
Original Data
Structural basis of the interaction between SETD2 methyltransferase and hnRNP L paralogs for governing co-transcriptional splicing Bhattacharya S, Wang S, Reddy D, Shen S, Zhang Y, Zhang N, Li H, Washburn MP, Florens L, Shi Y, Workman JL, Li F.
Nat Commun. 2021;12:6452. doi: 6410.1038/s41467-41021-26799-41463.
Original Data
Nucleotide Metabolism Behind Epigenetics Suganuma T, Workman JL.
Front Endocrinol (Lausanne). 2021;12:731648.
The SESAME complex regulates cell senescence through the generation of acetyl-CoA Chen W, Yu X, Wu Y, Tang J, Yu Q, Lv X, Zha Z, Hu B, Li X, Chen J, Ma L, Workman JL, Li S.
Nat Metab. 2021;3:983-1000. doi: 10.1038/s42255-021-00412-9.
The methyltransferase SETD2 couples transcription and splicing by engaging mRNA processing factors through its SHI domain Bhattacharya S, Levy MJ, Zhang N, Li H, Florens L, Washburn MP, Workman JL.
Nat Commun. 2021;12:1443. doi: 1410.1038/s41467-41021-21663-w.
Original Data
Metabolic regulation of telomere silencing by SESAME complex-catalyzed H3T11 phosphorylation Zhang S, Yu X, Zhang Y, Xue X, Yu Q, Zha Z, Gogol M, Workman JL, Li S.
Nat Commun. 2021;12:594. doi: 510.1038/s41467-41020-20711-41461.
2020
The plasticity of the pyruvate dehydrogenase complex confers a labile structure that is associated with its catalytic activity Lee J, Oh S, Bhattacharya S, Zhang Y, Florens L, Washburn MP, Workman JL.
PLos One. 2020;15:e0243489. doi: 10.1371/journal.pone.0243489.
Original Data
Driving Integrative Structural Modeling with Serial Capture Affinity Purification Liu X, Zhang Y, Wen Z, Hao Y, Banks CAS, Lange JJ, Slaughter BD, Unruh JR, Florens L, Abmayr SM, Workman JL, Washburn MP.
Proc Natl Acad Sci U S A. 2020;117:31861-31870.
Original Data
b-Catenin and Associated Proteins Regulate Lineage Differentiation in Ground State Mouse Embryonic Stem Cells Tao F, Soffers J, Hu D, Chen S, Gao X, Zhang Y, Zhao C, Smith SE, Unruh JR, Zhang D, Tsuchiya D, Venkatraman A, Zhao M, Li Z, Qian P, Parmely T, He XC, Washburn M, Florens L, Perry JM, Zeitlinger J, Workman J, Li L.
Stem Cell Rep. 2020;15:662-676.
Original Data
SAGA Structures Provide Mechanistic Models for Gene Activation Soffers JHM, Popova VV, Workman JLW.
Trends Biochem Sci. 2020;45:547-549.
When histones are under glucose starvation Lee J, Oh S, Abmayr SM, Workman JL.
J Biosci. 2020;45:e17.
2019
Identification of a nuclear localization signal and importin beta members mediating NUAK1 nuclear import inhibited by oxidative stress Palma M, Riffo EN, Suganuma T, Washburn MP, Workman JL, Pincheira R, Castro AF.
J Cell Biochem. 2019;120:16088-16107.
Original Data
Set1-catalyzed H3K4 trimethylation antagonizes the HIR/Asf1/Rtt106 repressor complex to promote histone gene expression and chronological life span
Mei Q, Xu C, Gogol M, Tang J, Chen W, Yu X,
Workman JL, Li S.
Nucleic Acids Res. 2019;47:3434-3449.
Characterization of a metazoan ADA acetyltransferase complex Soffers JHM, Li X, Saraf A, Seidel CW, Florens L, Washburn MP, Abmayr SM,
Workman JL.
Nucleic Acids Res. 2019;47:3383-3394.
Original Data
Histone lysine de-beta-hydroxybutyrylation by SIRT3 Abmayr SM,
Workman JL.
Cell Res. 2019;29:694-695.
NSL complex acetylates Lamin A/C Popova VV,
Workman JL.
Nat Cell Biol. 2019;21:1177-1178.
Rebooting the Epigenome: Erasure of Parental Histone Marks for Establishing the Regulatory Landscape in Zygotes Bhattacharya S,
Workman JL. [published ahead of print October 23 2019].
Biochemistry. 2019.
Exogenous pyruvate represses histone gene expression and inhibits cancer cell proliferation via the NAMPT-NAD+-SIRT1 pathway Ma R, Wu Y, Zhai Y, Hu B, Ma W, Yang W, Yu Q, Chen Z,
Workman JL, Yu X, Li S.
Nucleic Acids Res. 2019;47:11132-11150.
Glycolysis regulates gene expression by promoting the crosstalk between H3K4 trimethylation and H3K14 acetylation in Saccharomyces cerevisiaeY Wu, S Zhang, X Gong, Q Yu, Y Zhang, M Luo, X Zhang,
JL Workman, X Yu, S Li.
Journal of Genetics and Genomics Yi chuan xue bao 46 (12), 561 (2019). DOI: 10.1016/j.jgg.2019.11.007
2018
MPTAC Determines APP Fragmentation via Sensing Sulfur Amino Acid Catabolism Suganuma T, Swanson SK, Gogol M, Garrett TJ, Conkright-Fincham J, Florens L, Washburn MP,
Workman JL.
Cell Rep. 2018;24:1585-1596.
Original Data
Histone H3 threonine 11 phosphorylation by Sch9 and CK2 regulates chronological lifespan by controlling the nutritional stress response. Oh S, Suganuma T, Gogol MM,
Workman JL.
eLife. 2018;7:e36157. doi: 36110.37554/eLife.36157.
Original Data
Targeting BAF-perturbed cancers
Reddy D,
Workman JL.
Nat Cell Biol. 2018;20:1332-1333.
Chromatin and Metabolism Suganuma T,
Workman JL.
Annu Rev Biochem. 2018;87:27-49.
2017
Enzymatic modules of the SAGA chromatin-modifying complex play distinct roles in Drosophila gene expression and development Li X, Seidel CW, Szerszen LT, Lange JJ,
Workman JL, Abmayr SM.
Genes Dev. 2017;31:1588-1600.
Original Data
Myeloid Leukemia Factor acts in a chaperone complex to regulate transcription factor stability and gene Dyer JO, Dutta A, Gogol M, Weake VM, Dialynas G, Wu X, Seidel C, Zhang Y, Florens L, Washburn MP, Abmayr SM,
Workman JL.
J Mol Biol. 2017;429:2093-2107.
Original Data
Composition and Function of Mutant Swi/Snf Complexes Dutta A, Sardiu M, Gogol M, Gilmore J, Zhang D, Florens L, Abmayr SM, Washburn MP,
Workman JL.
Cell Rep. 2017;18:2124-2134.
Original Data
Chromatin remodeler Fun30Fft3 induces nucleosome disassembly to facilitate RNA polymerase II elongation Lee J, Shik Choi E, David Seo H, Kang K, Gilmore JM, Florens L, Washburn MP, Choe J,
Workman JL, Lee D.
Nat Commun. 2017;8:14527. doi: 14510.11038/ncomms14527.
Original Data
Role of Nhp6 and Hmo1 in SWI/SNF occupancy and nucleosome landscape at gene regulatory regions Hepp MI, Smolle M, Gidi C, Amigo R, Valenzuela N, Arriagada A, Maureira A, Gogol MM, Torrejon M,
Workman JL, Gutierrez JL.
Biochim Biophys Acta. 2017;1860:316-326.
Original Data
2016
Selective suppression of antisense transcription by Set2-mediated H3K36 methylation Venkatesh S, Li H, Gogol MM,
Workman JL.
Nat Commun. 2016;7:e13610. doi: 13610.11038/ncomms13610.
Original Data
USP44 Is an Integral Component of N-CoR that Contributes to Gene Repression by Deubiquitinating Histone H2BUSP44 Is an Integral Component of N-CoR that Contributes to Gene Repression by Deubiquitinating Histone H2B Lan X, Atanassov BS, Li W, Zhang Y, Florens L, Mohan RD, Galardy PJ, Washburn MP,
Workman JL, Dent SY.
Cell Rep. 2016;17:2382-2393.
Original Data
Limiting PCNA-unloading at the G1/S transition Huang F, Abmayr SM,
Workman JL. Limiting PCNA-unloading at the G1/S transition.
Cell Cycle. 2016;15:3001-3002.
Cytoplasmic ATXN7L3B Interferes with Nuclear Functions of the SAGA Deubiquitinase ModuleLi W, Atanassov BS, Lan X, Mohan RD, Swanson SK, Farria AT, Florens L, Washburn MP,
Workman JL, Dent SY.
Mol Cell Biol. 2016;36:2855-2866.
Original Data
Egf signaling directs neoblast repopulationby regulating asymmetric cell division in planarians Lei K, Thi-Kim Vu H, Mohan RD, McKinney SA, Seidel CW, Alexander R, Gotting K,
Workman JL, Sánchez Alvarado A.
Dev Cell. 2016;38:413-429.
Original Data
WDR76 Co-Localizes with Heterochromatin Related Proteins and Rapidly Responds to DNA Damage. Gilmore JM, Sardiu ME, Groppe BD, Thornton JL, Liu X, Dayebgadoh G, Banks CA, Slaughter BD, Unruh JR,
Workman JL, Florens L, Washburn MP.
PloS One. 2016;11:e0155492. doi: 0155410.0151371/journal.pone.0155492.
Original Data
The Enok acetyltransferase complex interacts with Elg1 and negatively regulates PCNA unloading to promote the G1/S transition Huang F, Saraf A, Florens L, Kusch T, Swanson SK, Szerszen LT, Li G, Dutta A, Washburn MP, Abmayr SM,
Workman JL.
Genes Dev. 2016;30:1198-1210.
Original Data
ATXN7L3 and ENY2 Coordinate Activity of Multiple H2B Deubiquitinases Important for Cellular Proliferation and Tumor Growth Atanassov BS, Mohan RD, Lan X, Kuang X, Lu Y, Lin K, McIvor E, Li W, Zhang Y, Florens L, Byrum SD, Mackintosh SG, Calhoun-Davis T, Koutelou E, Wang L, Tang DG, Tackett AJ, Washburn MP,
Workman JL, Dent SY.
Mol Cell. 2016;62:558-571.
Original Data
Reading and Interpreting the Histone Acylation Code Soffers JH, Li X, Abmayr SM,
Workman JL.
Bioinformatics. 2016;14:329-332.
Diverse Activities of Histone Acylations Connect Metabolism to Chromatin Function Dutta A, Abmayr SM,
Workman JL.
Mol Cell. 2016;63:547-552.
Regulation of KAT6 acetyltransferases and their roles in cell cycle progression, stem cell maintenance, and human disease Huang F, Abmayr SM,
Workman JL.
Mol Cell Biol. 2016;36:1900-1907.
CHROMATIN. It takes teamwork to modify chromatin. Workman JL.
Science. 2016;351:667.
Histone modification as a reflection of metabolism Suganuma T,
Workman JL.
Cell Cycle. 2016;15:481-482.
Moco biosynthesis and the ATAC acetyltransferase engage translation initiation by inhibiting latent PKR activity.Suganuma T, Swanson SK, Florens L, Washburn MP,
Workman JL.
J Mol Cell Biol. 2016;8(1):44–50.
CHROMATIN. It takes teamwork to modify chromatin. Workman JL.
Science. 2016;351(6274):667.
In Vitro Assembly of Nucleosomes for Binding/Remodeling Assays.Dutta A,
Workman JL.
Methods Mol Biol. 2017;1528:1–17.
Myeloid Leukemia Factor Acts in a Chaperone Complex to Regulate Transcription Factor Stability and Gene Expression.Dyer, JO, Dutta, A, Gogol, M, Weake, VM, Dialynas, G, Wu, X, Seidel, C, Zhang, Y, Florens, L, Washburn, MP, Abmayr, SM, &
Workman, JL J Mol Biol. 2017;429(13):2093–2107. doi:10.1016/j.jmb.2016.10.026
2015
2014
Histone acetyltransferase Enok regulates oocyte polarization by promoting expression of the actin nucleation factor spire. Huang F, Paulson A, Dutta A, Venkatesh S, Smolle M, Abmayr SM,
Workman JL.
Genes & Dev. 2014;28:2750-2763.
Original Data
Swi/Snf dynamics on stress-responsive genes is governed by competitive bromodomain interactions. Dutta A, Gogol M, Kim JH, Smolle M, Venkatesh S, Gilmore J, Florens L, Washburn MP,
Workman JL.
Genes Dev. 2014;29:2314-2330.
Original Data
Phosphorylation by Casein Kinase 2 Facilitates Psh1 Protein-assisted Degradation of Cse4 Protein. Hewawasam GS, Mattingly M, Venkatesh S, Zhang Y, Florens L,
Workman JL, Gerton JL.
J Biol Chem. 2014;289:29297-29309.
Original Data
Suberoylanilide Hydroxamic Acid (SAHA)-Induced Dynamics of a Human Histone Deacetylase Protein Interaction Network. Sardiu ME, Smith KT, Groppe BD, Gilmore JM, Saraf A, Egidy R, Peak A, Seidel CW, Florens L,
Workman JL, Washburn MP.
Mol Cell Proteomics. 2014;13:3114-3125.
Original Data
Nucleosome remodeling by the SWI/SNF complex is enhanced by yeast High Mobility Group Box (HMGB) proteins. Hepp MI, Alarcon V, Dutta A,
Workman JL, Gutierrez JL.
Biochim Biophys Acta. 2014;1839:764-772.
Loss of Drosophila Ataxin-7, a SAGA subunit, reduces H2B ubiquitination and leads to neural and retinal degeneration. Mohan RD, Dialynas G, Weake VM, Liu J, Martin-Brown S, Florens L, Washburn MP,
Workman JL, Abmayr SM.
Genes Dev. 2014;28:259-272.
Original Data
Drosophila models reveal novel insights into mechanisms underlying neurodegeneration. Mohan RD, Workman JL, Abmayr SM.
Fly. 2014;8:148-152.
The expanding role for chromatin and transcription in polyglutamine disease. Mohan RD, Abmayr SM,
Workman JL.
Curr Opin Genet Dev. 2014;26C:96-104.
Pulling complexes out of complex diseases: Spinocerebellar Ataxia 7. Mohan RD, Abmayr SM, Workman JL.
Rare Dis. 2014;2:e28859.
Recognizing methylated histone variant H3.3 to prevent tumors. Venkatesh S, Workman JL.
Cell Res. 2014;24:649-660.
RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing.Khan DH, Gonzalez C, Cooper C, Sun JM, Chen HY, Healy S, Xu W, Smith KT,
Workman JL, Leygue E, Davie JR.
Nucleic Acids Res. 2014;42(3):1656–1670.
2013
Acetylation regulates Jun protein turnover in Drosophila. Zhang D, Suganuma T,
Workman JL.
Biochim Biophys Acta. 2013;1829:1218-1224.
Original Data
Quantitative Proteomics Demonstrates That the RNA Polymerase II Subunits Rpb4 and Rpb7 Dissociate during Transcriptional Elongation. Mosley AL, Hunter GO, Sardiu ME, Smolle M,
Workman JL, Florens L, Washburn MP.
Mol Cell Proteomics. 2013;12:1530-1538.
Original Data
Remodelling without a power stroke. Dutta A,
Workman JL.
EMBO Rep. 2013;14:1030-1031.
Set2 mediated H3 lysine 36 methylation: regulation of transcription elongation and implications in organismal development. Venkatesh S,
Workman JL.
WIREs Dev Biol. 2013;2:685-700.
Nucleosome Remodeling and Epigenetics Becker PB,
Workman JL. Cold Spring Harb Perspect Biol. 2013;5:a017905. doi: 017910.011101/cshperspect.a017905.
Directing transcription to the right way. Huang F,
Workman JL.
Cell Res. 2013;23:1153-1154.
Chromatin and Signaling. Suganuma T,
Workman JL.
Curr Opin Cell Biol. 2013;25:322-326.
Transcription-associated histone modifications and cryptic transcription. Smolle M,
Workman JL.
Biochim Biophys Acta. 2013;1829:84-97.
UpSETing chromatin during non-coding RNA production. Venkatesh S,
Workman JL, Smolle M.
Epigenetics Chromatin. 2013;6:16.
reSETting chromatin during transcription elongation. Smolle M,
Workman JL, Venkatesh S.
Epigenetics
2012
Human Family with Sequence Similarity 60 Member A (FAM60A) Protein: a New Subunit of the Sin3 Deacetylase Complex. Smith KT, Sardiu ME, Martin-Brown SA, Seidel C, Mushegian A, Egidy R, Florens L, Washburn MP,
Workman JL.
Mol Cell Proteomics. 2012;11:1815-1828.
Chromatin remodelers Isw1 and Chd1 maintain chromatin structure during transcription by preventing histone exchange. Smolle M, Venkatesh S, Gogol MM, Li H, Zhang Y, Florens L, Washburn MP,
Workman JL.
Nat Stuct Mol Biol. 2012;19:884-892.
Set2 methylation of histone H3 lysine36 suppresses histone exchange on transcribed genes. Venkatesh S, Smolle M, Li H, Gogol MM, Saint M, Kumar S, Natarajan K,
Workman JL.
Nature 2012;489:452-455.
HP1a targets the Drosophila KDM4A demethylase to a subset of heterochromatic genes to regulate H3K36me3 levels. Lin C-H, Paulson A, Abmayr SM,
Workman JL.
PLoS One. 2012:e39758.
Original Data
Acetylation of the SUN protein Mps3 by Eco1 regulates its function in nuclear organization. Ghosh S, Gardner JM, Smoyer CJ, Friederichs JM, Unruh JR, Slaughter BD, Alexander R, Chisholm RD, Lee KK,
Workman JL, Jaspersen SL.
Mol Biol Cell. 2012;23:2546-2559.
Characterization of a highly conserved histone related protein, ydl156w, and its functional associations using quantitative proteomic analyses. Gilmore JM, Sardiu ME, Venkatesh S, Stutzman B, Peak A, Seidel CW,
Workman JL, Florens L, Washburn MP.
Mol Cell Proteomics. 2012;11:M111 011544.
Human CCAAT/Enhancer-Binding Protein beta Interacts with Chromatin Remodeling Complexes of the Imitation Switch Subfamily. Steinberg XP, Hepp MI, Fernandez Garcia Y, Suganuma T, Swanson SK, Washburn M,
Workman JL, Gutierrez JL.
Biochemistry. 2012;51:952-962.
A metazoan ATAC acetyltransferase subunit that regulates MAP kinase signaling is related to an ancient molybdopterin synthase component. Suganuma T, Mushegian A, Swanson SK, Florens L, Washburn MP,
Workman JL.
Mol Cell Proteomics. 2012;11:90-99.
Original Data
Histone density is maintained during nucleosomal transcription mediated by RSC and NAP1 in vitro. Kuryan BG, Kim J, Tran NNH, Lombardo SR, Venkatesh S,
Workman JL, Carey M.
PNAS. 2012;109:1931-1936.
Nucleosome Positioning: Multiple Mechanisms toward a Unifying Goal. Dutta A,
Workman JL. [published ahead of print October 16 2012].
Mol Cell. 2012;48:1-2.
Holding on through DNA Replication: Histone Modification or Modifier? Abmayr SM,
Workman JL.
Cell. 2012;150:875-877.
Chromatin proteins: key responders to stress. Smith KT,
Workman JL.
PLoS Biol. 2012;10:e1001371.
MAP kinases and histone modification. Suganuma T,
Workman JL.
J Mol Cell Biol. 2012;4:348-350.
SAGA function in tissue-specific gene expression. Weake VM, Workman JL.
Trends Cell Biol. 2012;22:177-184.
2011
Structure and nucleosome interaction of the yeast NuA4 and Piccolo-NuA4 histone acetyltransferase complexes. Chittuluru JR, Chaban Y, Monnet-Saksouk J, Carrozza MJ, Sapountzi V, Selleck W, Huang J, Utley RT, Cramet M, Allard S, Cai G,
Workman JL, Fried MG, Tan S, Cote J, Asturias FJ.
Nat Struct Mol Biol. 2011;18:1196-1203.
Post-transcription initiation function of the ubiquitous SAGA complex in tissue-specific gene activation. Weake VM, Dyer JO, Seidel C, Box A, Swanson SK, Peak A, Florens L, Washburn MP, Abmayr SM,
Workman JL.
Genes Dev. 2011;25:1499-1509.
Combinatorial depletion analysis to assemble the network architecture of the SAGA and ADA chromatin remodeling complexes. Lee KK, Sardiu ME, Swanson SK, Gilmore JM, Torok M, Grant PA, Florens L,
Workman JL, Washburn MP.
Mol Syst Biol. 2011;7:503.
Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation Bian C, Xu C, Ruan J, Lee KK, Burke TL, Tempel W, Barsyte D, Li J, Wu M, Zhou BO, Fleharty BE, Paulson A, Allali-Hassani A, Zhou JQ, Mer G, Grant PA,
Workman JL, Zang J, Min
J. EMBO J. 2011;30:2829-2842.
Original Data
Highly reproducible label free quantitative proteomic analysis of RNA polymerase complexes. Mosley AL, Sardiu ME, Pattenden SG,
Workman JL, Florens L, Washburn MP.
Mol Cell Proteomics. 2011;10:M110 000687.
A role for Snf2-related nucleosome-spacing enzymes in genome-wide nucleosome organization. Gkikopoulos T, Schofield P, Singh V, Pinskaya M, Mellor J, Smolle M,
Workman JL, Barton GJ, Owen-Hughes T.
Science. 2011;333:1758-1760.
Signaling through Chromatin: Setting the Scene at Kinetochores. Smolle M,
Workman JL.
Cell. 2011;146:671-672.
Signals and combinatorial functions of histone modifications. Suganuma T,
Workman JL.
Annu Rev Biochem. 2011;80:473-499.
Suppression of cryptic intragenic transcripts is required for embryonic stem cell self-renewal. Lin CH,
Workman JL.
EMBO J. 2011;30:1420-1421.
Readers of histone modifications. Yun M, Wu J,
Workman JL, Li B.
Cell Res. 2011;21:564-578.
HP1c casts light on dark matter. Kwon SH,
Workman JL.
Cell Cycle. 2011;10:625-630.
The proteasome and its regulatory roles in gene expression. Kwak J,
Workman JL, Lee D.
Biochim Biophys Acta. 2011;1809:88-96.
The changing faces of HP1: From heterochromatin formation and gene silencing to euchromatic gene expression: HP1 acts as a positive regulator of transcription. Kwon SH,
Workman JL.
BioEssays. 2011;33:280-289.
2010
Gcn5 regulates the dissociation of SWI/SNF from chromatin by acetylation of Swi2/Snf2. Kim JH, Saraf A, Florens L, Washburn M,
Workman JL.
Genes Dev. 2010;24:2766-2771.
Psh1 Is an E3 Ubiquitin Ligase that Targets the Centromeric Histone Variant Cse4. Hewawasam G, Shivaraju M, Mattingly M, Venkatesh S, Martin-Brown S, Florens L,
Workman JL, Gerton JL.
Mol Cell. 2010;40:444-454.
Heterochromatin protein 1 (HP1) connects the FACT histone chaperone complex to the phosphorylated CTD of RNA polymerase II. Kwon SH, Florens L, Swanson SK, Washburn MP, Abmayr SM,
Workman JL.
Genes Dev. 2010;24:2133-2145.
Features of cryptic promoters and their varied reliance on bromodomain-containing factors. Pattenden SG, Gogol MM,
Workman JL.
PLoS One. 2010;5:e12927.
The ATAC Acetyltransferase Complex Coordinates MAP Kinases to Regulate JNK Target Genes. Suganuma T, Mushegian A, Swanson SK, Abmayr SM, Florens L, Washburn MP,
Workman JL.
Cell. 2010;142:726-736.
Phosphorylated Pol II CTD recruits multiple HDACs, including Rpd3C(S), for methylation-dependent deacetylation of ORF nucleosomes. Govind CK, Qiu H, Ginsburg DS, Ruan C, Hofmeyer K, Hu C, Swaminathan V,
Workman JL, Li B, Hinnebusch AG.
Mol Cell. 2010;39:234-246.
Deacetylase Inhibitors Dissociate the Histone-Targeting ING2 Subunit from the Sin3 Complex. Smith KT, Martin-Brown SA, Florens L, Washburn MP,
Workman JL.
Chem Biol. 2010;17:65-74.
Features of the PHF8/KIAA1718 histone demethylase. Suganuma T,
Workman JL.
Cell Res. 2010;20:861-862.
Inducible gene expression: diverse regulatory mechanisms. Weake VM,
Workman JL.
Nat Rev Genet. 2010;11:426-437.
Histone acetylation in heterochromatin assembly. Kim JH,
Workman JL.
Genes Dev. 2010;24:738-740.
Rere controls retinoic acid signalling and somite bilateral symmetry. Vilhais-Neto GC, Maruhashi M, Smith KT, Vasseur-Cognet M, Peterson AS,
Workman JL, Pourquie O.
Nature. 2010;463:953-957.
WD40 repeats arrange histone tails for spreading of silencing. Suganuma T,
Workman JL.
J Mol Cell Biol. 2010;2:81-83.
2009
A novel histone fold domain-containing protein that replaces TAF6 in Drosophila SAGA is required for SAGA-dependent gene expression. Weake VM, Swanson SK, Mushegian A, Florens L, Washburn MP, Abmayr SM,
Workman JL.
Genes Dev. 2009;23:2818-2823.
Determining protein complex connectivity using a probabilistic deletion network derived from quantitative proteomics. Sardiu ME, Gilmore JM, Carrozza MJ, Li B,
Workman JL, Florens L, Washburn MP.
PLoS One. 2009;4:e7310.
Cse4 is part of an octameric nucleosome in budding yeast. Camahort R, Shivaraju M, Mattingly M, Li B, Nakanishi S, Zhu D, Shilatifard A,
Workman JL, Gerton JL.
Mol Cell. 2009;35:794-805.
Activator-binding domains of the SWI/SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo. Ferreira ME, Prochasson P, Berndt KD,
Workman JL, Wright APH.
FEBS J. 2009;276:2557-2565.
Rtr1 is a CTD phosphatase that regulates RNA polymerase II during the transition from serine 5 to serine 2 phosphorylation Mosley AL, Pattenden SG, Carey M, Venkatesh S, Gilmore JM, Florens L,
Workman JL, Washburn MP.
Mol Cell. 2009;34:168-178.
Histone H3 lysine 36 dimethylation (H3K36me2) is sufficient to recruit the Rpd3s histone deacetylase complex and to repress spurious transcription. Li B, Jackson J, Simon MD, Fleharty B, Gogol M, Seidel C,
Workman JL, Shilatifard A.
J Biol Chem. 2009;284:7970-7976.
Yeast Sgf73/Ataxin-7 serves to anchor the deubiquitination module into both SAGA and Slik(SALSA) HAT complexes. Lee KK, Swanson SK, Florens L, Washburn MP,
Workman JL.
Epigenetics Chromatin. 2009;2:2.
Introducing the acetylome. Smith KT,
Workman JL.
Nat Biotechnol. 2009;27:917-919.
Histone Ubiquitination. Weake VM,
Workman JL. In: RA Bradshaw, and EA Dennis, eds.
Handbook of Cell Signaling. 2nd ed: Oxford: Academic Press; 2009:2449-2460.
Hit and run: X marks the spot! Weake VM,
Workman JL.
Nat Struct Mol Biol. 2009;16:801-803.
Histone deacetylase inhibitors: Anticancer compounds Smith KT,
Workman JL.
Int J Biochem Cell Biol. 2009;41:21-25.
2008
Heterochromatin Protein 1a stimulates Histone H3 Lysine 36 Demethylation by the Drosophila KDM4A Demethylase Lin CH, Li B, Swanson S, Zhang Y, Florens L, Washburn MP, Abmayr SM,
Workman JL.
Mol Cell.2008;32:696-706.
ATAC is a double histone acetyltransferase complex that stimulates nucleosome slidingSuganuma T, Gutierrez JL, Li B, Florens L, Swanson SK, Washburn MP, Abmayr SM,
Workman JL.
Nat Struct Mol Biol.2008;15:364-372.
SAGA-mediated H2B deubiquitination controls the development of neuronal connectivity in the Drosophila visual system Weake VM, Lee KK, Guelman S, Lin CH, Seidel C, Abmayr SM,
Workman JL.
Embo J.2008;27:394-405.
The Heterochromatin Protein 1 (HP1) Family: Put Away a Bias toward HP1 Kwon SH,
Workman JL.
Mol Cells.2008;26:217-227.
Diverse functions of WD40 repeat proteins in histone recognition Suganuma T, Pattenden SG,
Workman JL.
Genes Dev.2008;22:1265-1268.
Crosstalk among Histone Modifications Suganuma T,
Workman JL.
Cell.2008;135:604-607.
Clearing the Way for Unpaused Polymerases Weake VM,
Workman JL.
Cell.2008;134:16-18.
Histone Ubiquitination: Triggering Gene Activity Weake VM,
Workman JL.
Mol Cell.2008;29:653-663.
2007
Activation domains drive nucleosome eviction by SWI/SNF Gutierrez JL, Chandy M, Carrozza MJ,
Workman JL.
Embo J.2007;26:730-740.
MSL Complex Is Attracted to Genes Marked by H3K36 Trimethylation Using a Sequence-Independent Mechanism Larschan E, Alekseyenko AA, Gortchakov AA, Peng S, Li B, Yang P,
Workman JL, Park PJ, Kuroda MI.
Mol Cell.2007;28:121-133.
Combined Action of PHD and Chromo Domains Directs the Rpd3S HDAC to Transcribed Chromatin Li B, Gogol M, Carey M, Lee D, Seidel C,
Workman JL.
Science.2007;316:1050-1054.
Infrequently transcribed long genes depend on the Set2/Rpd3S pathway for accurate transcription Li B, Gogol M, Carey M, Pattenden SG, Seidel C,
Workman JL.
Genes Dev.2007;21:1422-1430.
New Nomenclature for Chromatin-Modifying Enzymes Allis CD, Berger SL, Cote J, Dent S, Jenuwien T, Kouzarides T, Pillus L, Reinberg D, Shi Y, Shiekhattar R, Shilatifard A,
Workman J, Zhang Y.
Cell.2007;131:633-636.
Histone Variants and Complexes Involved in Their Exchange Kusch T,
Workman JL.
Chromatin and Disease: Subcellular Biochemistry. New York: Springer 2007;41:91-110.
Histone acetyltransferase complexes: one size doesn't fit all Lee KK,
Workman JL.
Nat Rev Mol Cell Biol.2007;8:284-295.
The Role of Chromatin during Transcription Li B, Carey M,
Workman JL.
Cell.2007;128:707-719.
2006
RSC Exploits Histone Acetylation to Abrogate the Nucleosomal Block to RNA Polymerase II Elongation Carey M, Li B,
Workman JL.
Mol Cell.2006;24:481-487.
SWI/SNF Displaces SAGA-Acetylated Nucleosomes Chandy M, Gutierrez JL, Prochasson P,
Workman JL.
Eukaryot Cell.2006;5:1738-1747.
Host Cell Factor and an Uncharacterized SANT Domain Protein Are Stable Components of ATAC, a Novel dAda2A/dGcn5-Containing Histone Acetyltransferase Complex in Drosophila Guelman S, Suganuma T, Florens L, Swanson SK, Kiesecker CL, Kusch T, Anderson S, Yates JR, 3rd, Washburn MP, Abmayr SM,
Workman JL.
Mol Cell Biol.2006;26:871-882.
The Essential Gene wda Encodes a WD40 Repeat Subunit of Drosophila SAGA Required for Histone H3 Acetylation Guelman S, Suganuma T, Florens L, Weake V, Swanson SK, Washburn MP, Abmayr SM,
Workman JL.
Mol Cell Biol.2006;26:7178-7189.
SAS-mediated acetylation of histone H4 Lys 16 is required for H2A.Z incorporation at subtelomeric regions in Saccharomyces cerevisiae Shia WJ, Li B,
Workman JL. Genes Dev.2006;20:2507-2512.
Analyzing chromatin remodeling complexes using shotgun proteomics and normalized spectral abundance factors Florens L, Carozza MJ, Swanson SK, Fournier M, Coleman MK,
Workman JL, Washburn MP.
Methods.2006;40:303-311.
Cre/
loxP Strategies
Shia WJ, Pattenden SG,
Workman JL, Sauer B. In:D Ganten, and K Ruckpaul, eds.
Encyclopedic Reference of Genomics and Proteomics in Molecular Medicine. Berlin ; New York: Springer; 2006;2:2090.
Histone H4 lysine 16 acetylation breaks the genome's silence Shia WJ, Pattenden SG,
Workman JL.
Genome Biol.2006;7:217.
Nucleosome displacement in transcription Workman JL.
Genes Dev.2006
2005
the Mammalian YL1 Protein Is a Shared Subunit of the TRRAP/TIP60 Histone Acetyltransferase and SRCAP Complexes Cai Y, Jin J, Florens L, Swanson SK, Kusch T, Li B,
Workman JL, Washburn MP, Conaway RC, Conaway JW.
J Biol Chem.2005:13665-13670.
Stable incorporation of sequence specific repressors Ash1 and Ume6 into the Rpd3L complex Carrozza MJ, Florens L, Swanson SK, Shia WJ, Anderson S, Yates J, Washburn MP,
Workman JL.
Biochim Biophys Acta.2005;1731:77-87; discussion 75-76.
Histone H3 Methylation by Set2 Directs Deacetylation of Coding Regions by Rpd3S to Suppress Spurious Intragenic Transcription Carrozza MJ, Li B, Florens L, Suganuma T, Swanson SK, Lee KK, Shia WJ, Anderson S, Yates J, Washburn MP,
Workman JL.
Cell.2005;123:581-592.
Yeast Recombination Enhancer Is Stimulated by Transcription Activation Ercan S, Reese JC,
Workman JL, Simpson RT.
Mol Cell Biol.2005;25:7976-7987.
Mechanism of Transcription Factor Recruitment by Acidic Activators Ferreira ME, Hermann S, Prochasson P,
Workman JL, Berndt KD, Wright AP.
J Biol Chem.2005.
A Mammalian Chromatin Remodeling Complex with Similarities to the Yeast INO80 Complex Jin J, Cai Y, Yao T, Gottschalk AJ, Florens L, Swanson SK, Gutierrez JL, Coleman MK,
Workman JL, Mushegian A, Washburn MP, Conaway RC, Conaway JW.
J Biol Chem.2005;280:41207-41212.
The Proteasome Regulatory Particle Alters the SAGA Coactivator to Enhance Its Interactions with Transcriptional ActivatorsLee KK, Florens L, Swanson SK, Washburn MP,
Workman JL, Lee D, Ezhkova E, Li B, Pattenden SG, Tansey WP, Workman JL.
Cell.2005;123:423-436.
The Deubiquitylation Activity of Ubp8 Is Dependent upon Sgf11 and Its Association with the SAGA Complex
Lee KK, Florens L, Swanson SK, Washburn MP,
Workman JL.
Mol Cell Biol.2005;25:1173-1182.
Preferential occupancy of histone variant H2AZ at inactive promoters influences local histone modifications and chromatin remodeling Li B, Pattenden SG, Lee D, Gutierrez J, Chen J, Seidel C, Gerton J,
Workman JL.
Proc Natl Acad Sci U S A.2005.
The HIR corepressor complex binds to nucleosomes generating a distinct protein/DNA complex resistant to remodeling by SWI/SNF Prochasson P, Florens L, Swanson SK, Washburn MP,
Workman JL.
Genes Dev.2005;19:2534-2539.
The DNA Repair Protein yKu80 Regulates the Function of Recombination Enhancer during Yeast Mating Type Switching Ruan C,
Workman JL, Simpson RT.
Mol Cell Biol.2005;25:8476-8485.
Characterization of the Yeast Trimeric-SAS Acetyltransferase Complex Shia WJ, Osada S, Florens L, Swanson SK, Washburn MP,
Workman JL.
J Biol Chem.2005;280:11987-11994.
In and out: histone variant exchange in chromatin Jin J, Cai Y, Li B, Conaway RC,
Workman JL, Conaway JW, Kusch T.
Trends Biochem Sci.2005;30:680-687.
Histones: should I stay or should I go? \
Li B, Ruan C,
Workman JL.
Genome Biol.2005;6:306.
Chromatin dynamics rule the genome Pattenden SG, Chandy MJ, Gutierrez JL,
Workman JL.
Genome Biol.2005;6:355.
2004
Acetylation by Tip60 Is Required for Selective Histone Variant Exchange at DNA Lesions Kusch T, Florens L, Macdonald WH, Swanson SK, Glaser RL, Yates Iii JR, Abmayr SM, Washburn MP,
Workman JL.
Science.2004;306:2084-2087.
Proteomic analysis of chromatin-modifying complexes in Saccharomyces cerevisiae identifies novel subunits Lee KK, Prochasson P, Florens L, Swanson SK, Washburn MP,
Workman JL.
Biochem Soc Trans.2004;32:899-903.
Global Position and Recruitment of HATs and HDACs in the Yeast Genome Robert F, Pokholok DK, Hannett NM, Rinaldi NJ, Chandy M, Rolfe A,
Workman JL, Gifford DK, Young RA.
Mol Cell.2004;16:199-209.
Global nucleosome distribution and the regulation of transcription in yeast Ercan S, Carrozza MJ,
Workman JL.
Genome Biol.2004;5:243.
Histone H3 variants and modifications on transcribed genes Workman JL, Abmayr SM.
Proc Natl Acad Sci U S A. 2004;101:1429-1430.
2003
Transcription factors prominently in Lasker Award to Roeder.Abmayr SM,
Workman JL.
Cell (2003);115:243-6. PMID:14636549
Assay of activator recruitment of chromatin-modifying complexes.Carrozza MJ, Hassan AH,
Workman JL.
Methods Enzymol (2003);371:536-44.
Repairing nucleosomes during transcription. Carrozza MJ, Kusch T,
Workman JL.
Nat Struct Biol (2003);10:879-80.
The diverse functions of histone acetyltransferase complexes.Carrozza MJ, Utley RT,
Workman JL, Cote J.
Trends Genet (2003);19:321-9.
Kusch T, Guelman S, Abmayr SM,
Workman JL. Two Drosophila Ada2 homologues function in different multiprotein complexes.
Mol Cell Biol (2003);23:3305-19. PMCID:
PMC153191 Li B, Howe L, Anderson S, Yates JR, 3rd,
The Set2 histone methyltransferase functions through the phosphorylated carboxyl-terminal domain of RNA polymerase II.
Workman JL.
J Biol Chem (2003);278:8897-903.
Opposite role of yeast ING family members in p53-dependent transcriptional activation.Nourani A, Howe L, Pray-Grant MG,
Workman JL, Grant PA, Cote J.
J Biol Chem (2003);278:19171-5.
Targeting activity is required for SWI/SNF function in vivo and is accomplished through two partially redundant activator-interaction domains.
Prochasson P, Neely KE, Hassan AH, Li B,
Workman JL.
Mol Cell (2003);12:983-90. PMID:14580348
Sas4 and Sas5 are required for the histone acetyltransferase activity of Sas2 in the SAS complex.Sutton A, Shia WJ, Band D, Kaufman PD, Osada S,
Workman JL, Sternglanz R.
J Biol Chem (2003);278:16887-92.
2002
Gal80 confers specificity on HAT complex interactions with activators.
Carrozza MJ, John S, Sil AK, Hopper JE,
Workman JL.
J Biol Chem (2002);277:24648-52.
Chromosome and expression mechanisms: a year dominated by histone modifications, transitory and remembered.
Elgin SC,
Workman JL.
Curr Opin Genet Dev (2002);12:127-9. PMID:11893483
Recruitment of Gcn5-containing complexes during c-Myc-dependent gene activation. Structure and function aspects.Flinn EM, Wallberg AE, Hermann S, Grant PA,
Workman JL, Wright AP.
J Biol Chem (2002);277:23399-406.
Function and selectivity of bromodomains in anchoring chromatin-modifying complexes to promoter nucleosomes.Hassan AH, Prochasson P, Neely KE, Galasinski SC, Chandy M, Carrozza MJ,
Workman JL.
Cell (2002);111:369-79.
Yng1p modulates the activity of Sas3p as a component of the yeast NuA3 Hhistone acetyltransferase complex.Howe L, Kusch T, Muster N, Chaterji R, Yates JR, 3rd,
Workman JL.
Mol Cell Biol (2002);22: 5047-53.
Transcription activator interactions with multiple SWI/SNF subunits.Neely KE, Hassan AH, Brown CE, Howe L,
Workman JL.
Mol Cell Biol 2002;22:1615-25. PMCID:
PMC135607
The complexity of chromatin remodeling and its links to cancer.Neely KE,
Workman JL.
Biochim Biophys Acta (2002);1603:19-29.
Histone acetylation and chromatin remodeling: which comes first?Neely KE,
Workman JL.
Mol Genet Metab (2002);76:1-5.
The novel SLIK histone acetyltransferase complex functions in the yeast retrograde response pathway.Pray-Grant MG, Schieltz D, McMahon SJ, Wood JM, Kennedy EL, Cook RG,
Workman JL, Yates JR, 3rd, Grant PA.
Mol Cell Biol (2002);22:8774-86.
2001
Recruitment of HAT complexes by direct activator interactions with the ATM-related Tra1 subunit.Brown CE, Howe L, Sousa K, Alley SC, Carrozza MJ, Tan S,
Workman JL.
Science (2001);292:2333-7.
Promoter targeting of chromatin-modifying complexes.Hassan AH, Neely KE, Vignali M, Reese JC,
Workman JL.
Front Biosci (2001);6:D1054-64.
Histone acetyltransferase complexes stabilize swi/snf binding to promoter nucleosomes.Hassan AH, Neely KE,
Workman JL.
Cell (2001);104:817-27.
Histone H3 specific acetyltransferases are essential for cell cycle progression. Howe L, Auston D, Grant P, John S, Cook RG,
Workman JL, Pillus L.
Genes Dev (2001);15:3144-54.
The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1.Osada S, Sutton A, Muster N, Brown CE, Yates JR, 3rd, Sternglanz R,
Workman JL.
Genes Dev (2001);15:3155-68.
2000
The many HATs of transcription coactivators. Brown CE, Lechner T, Howe L,
Workman JL.
Trends Biochem Sci (2000);25:15-9. PMID:10637607
The something about silencing protein, Sas3, is the catalytic subunit of NuA3, a yTAF(II)30-containing HAT complex that interacts with the Spt16 subunit of the yeast CP (Cdc68/Pob3)-FACT complex. John S, Howe L, Tafrov ST, Grant PA, Sternglanz R,
Workman JL.
Genes Dev (2000);14:1196-208.
Sds3 (suppressor of defective silencing 3) is an integral component of the yeast Sin3[middle dot]Rpd3 histone deacetylase complex and is required for histone deacetylase activity.Lechner T, Carrozza MJ, Yu Y, Grant PA, Eberharter A, Vannier D, Brosch G, Stillman DJ, Shore D,
Workman JL.
J Biol Chem (2000);275:40961-6. DOI:
10.1074/jbc.M005730200
Promoter targeting and chromatin remodeling by the SWI/SNF complex. Peterson CL,
Workman JL.
Curr Opin Genet Dev (2000);10:187-92. PMID:10753786
ATP-dependent chromatin-remodeling complexes.Vignali M, Hassan AH, Neely KE,
Workman JL.
Mol Cell Biol (2000);20:1899-910. PMCID:
PMC110808
Distribution of acetylated histones resulting from Gal4-VP16 recruitment of SAGA and NuA4 complexes.Vignali M, Steger DJ, Neely KE,
Workman JL.
EMBO J (2000);19:2629-40. DOI:
10.1093/emboj/19.11.2629
Recruitment of the SWI-SNF chromatin remodeling complex as a mechanism of gene activation by the glucocorticoid receptor tau1 activation domain.
Wallberg AE, Neely KE, Hassan AH, Gustafsson JA,
Workman JL, Wright AP.
Mol Cell Biol (2000);20:2004-13. PMCID:
PMC110817
1990s
An upstream transcription factor, USF (MLTF), facilitates the formation of preinitiation complexes during in vitro chromatin assembly.
Workman JL, Roeder RG, Kingston RE.
EMBO J (1990);9:1299-308. P
Preparation of nuclear extracts from cultured cells.Ambayr SM,
Workman JL. In
Current Protocols in Molecular Biology. Ausubel FM, Brent R, Kingston RE, Moore DD, Siedman JG, Smith JA, Strubl K. eds. Greene Publishing Associates/Wiley Interscience, New York. (1990).
Facilitated binding of GAL4 and heat shock factor to nucleosomal templates: differential function of DNA-binding domains.
Taylor IC,
Workman JL, Schuetz TJ, Kingston RE.
Genes Dev (1991);5:1285-98. PMID:2065977
Activation domains of stably bound GAL4 derivatives alleviate repression of promoters by nucleosomes.
Workman JL, Taylor IC, Kingston RE.
Cell (1991);64:533-44. PMID:1991320
Control of class II gene transcription during in vitro nucleosome assembly.
Workman JL, Taylor IC, Kingston RE, Roeder RG.
Methods Cell Biol (1991);35:419-47. PMID:1779863
Regulation of DNA replication in vitro by the transcriptional activation domain of GAL4-VP16.Cheng LZ,
Workman JL, Kingston RE, Kelly TJ.
Proc Natl Acad Sci U S A (1992);89:589-93.
Preparation of nuclear extracts from cultured cells.Ambayr SM,
Workman JL. In
Short Protocols in Molecular Biology. Ausubel FM, Brent R, Kingston RE, Moore DD, Siedman JG, Smith JA, Strubl K. eds. Greene Publishing Associates/Wiley Interscience, New York. (1992):12.1-12.5.
Nucleosome core displacement in vitro via a metastable transcription factor-nucleosome complex.
Workman JL, Kingston RE.
Science (1992);258:1780-4. PMID:1465613
Nucleosome displacement in transcription.Adams CC,
Workman JL.
Cell (1993);72:305-8. PMID:8431942
Nucleosome cores and histone H1 in the binding of GAL4 derivatives and the reactivation of transcription from nucleosome templates in vitro. Juan LJ, Walter PP, Taylor IC, Kingston RE,
Workman JL.
Cold Spring Harb Symp Quant Biol (1993);58:213-23. PMID:7956032
Multiple functions of nucleosomes and regulatory factors in transcription.
Workman JL, Buchman AR.
Trends Biochem Sci (1993);18:90-5. PMID:8480368
Nucleosome cores and histone H1 in the binding of GAL4 derivatives and the reactivation of transcription from nucleosome templates in vitro.
Juan LJ, Walter PP, Taylor ICA, Kingston RE,
Workman JL.
Cold Spring Harb Symp Quant Biol: DNA and Chromosomes. (1993);58:213-223. PMID:7956032
Transcription regulation of a chromatin template.Gilmour D, Buchman AR,
Workman JL. In:
Transcription Mechanism and Regulation. Conaway RC and Conaway JW eds. Raven Press, New York. (1994);515-34.
A histone-binding protein, nucleoplasmin, stimulates transcription factor binding to nucleosomes and factor-induced nucleosome disassembly.
Chen H, Li B,
Workman JL.
EMBO J (1994);13:380-90. PMCID:
PMC394819
Stimulation of GAL4 derivative binding to nucleosomal DNA by the yeast SWI/SNF complex.Cote J, Quinn J,
Workman JL, Peterson CL.
Science (1994);265: 3-60. PMID:8016655
Differential repression of transcription factor binding by histone H1 is regulated by the core histone amino termini.Juan LJ, Utley RT, Adams CC, Vettese-Dadey M,
Workman JL.
EMBO J (1994);13:6031-40.
Nucleosome binding by the constitutive transcription factor Sp1.Li B, Adams CC,
Workman JL.
J Biol Chem (1994);269:7756-63. PMID:8126002
Experimental analysis of chromatin function in transcription control.Owen-Hughes T,
Workman JL.
Crit Rev Eukaryot Gene Expr (1994);4: 403-41. PMID:7734837
Role of the histone amino termini in facilitated binding of a transcription factor, GAL4-AH, to nucleosome cores.Vettese-Dadey M, Walter P, Chen H, Juan LJ,
Workman JL.
Mol Cell Biol (1994);14:970-81.
Binding of disparate transcriptional activators to nucleosomal DNA is inherently cooperative.Adams CC,
Workman JL.
Mol Cell Biol (1995);15:1405-21.
Basic analysis of transcription factor binding to nucleosomes.Cote J, Utley RT,
Workman JL.
Methods Mol Genet (1995);6:108-28.
Experimental analysis of transcription factor/nucleosome interactions. Vettese-Dadey M, Adams CC, Cote J, Walter PP,
Workman JL.
Methods Mol Genet (1995);6:129-52.
Stimulation of transcription factor binding and histone displacement by nucleosome assembly protein 1 and nucleoplasmin requires disruption of the histone octamer.
Walter PP, Owen-Hughes TA, Cote J,
Workman JL.
Mol Cell Biol (1995);15:6178-87.
Mechanism and consequences of transcription factor binding to nucleosomes.Walter PP, Vettese-Dadey M, Cote J, Adams CC, Juan LF, Utley R,
Workman JL. In:
The Nucleus. AP Wolffe ed., JAI Press, Greenwich (1995);1:79-100.
The TAF(II)250 subunit of TFIID has histone acetyltransferase activity.Mizzen CA, Yang XJ, Kokubo T, Brownell JE, Bannister AJ, Owen-Hughes T,
Workman JL, Wang L, Berger SL, Kouzarides T, Nakatani Y, Allis CD.
Cell (1996);87:1261-70. PMID:8980232
Binding of the wheat basic leucine zipper protein EmBP-1 to nucleosomal binding sites is modulated by nucleosome positioning.
Niu X, Adams CC,
Workman JL, Guiltinan MJ.
Plant Cell (1996);8:1569-87.
Persistent site-specific remodeling of a nucleosome array by transient action of the SWI/SNF complex.Owen-Hughes T, Utley RT, Cote J, Peterson CL,
Workman JL. Science (1996);273:513-6.
Remodeling the chromatin structure of a nucleosome array by transcription factor-targeted trans-displacement of histones.
Owen-Hughes T,
Workman JL. EMBO J (1996);15:4702-12.
Remodeling chromatin structures for transcription: what happens to the histones?Steger DJ,
Workman JL. Bioessays (1996);18:875-84.
In vitro analysis of transcription factor binding to nucleosomes and nucleosome disruption/displacement. Utley RT, Owen-Hughes TA, Juan LJ, Cote J, Adams CC,
Workman JL.
Methods Enzymol (1996);274:276-91. PMID:8902812
Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro.Vettese-Dadey M, Grant PA, Hebbes TR, Crane- Robinson C, Allis CD,
Workman JL.
EMBO J (1996);15:2508-18.
Purification and biochemical heterogeneity of the mammalian SWI-SNF complex.Wang W, Cote J, Xue Y, Zhou S, Khavari PA, Biggar SR, Muchardt C, Kalpana GV, Goff SP, Yaniv M,
Workman JL, Crabtree GR.
EMBO J (1996);15:5370-82.
Yeast Gcn5 functions in two multisubunit complexes to acetylate nucleosomal histones: characterization of an Ada complex and the SAGA (Spt/Ada) complex.
Grant PA, Duggan L, Cote J, Roberts SM, Brownell JE, Candau R, Ohba R, Owen-Hughes T, Allis CD, Winston F, Berger SL,
Workman JL. Genes Dev (1997);11:1640-50. PMID:9224714
H1-mediated repression of transcription factor binding to a stably positioned nucleosome.Juan LJ, Utley RT, Vignali M, Bohm L,
Workman JL. J Biol Chem (1997);272:3635-40. PMID:9013616
Nucleosome binding by the polymerase I transactivator upstream binding factor displaces linker histone H1.Kermekchiev M, Workman JL, Pikaard CS.
Mol Cell Biol (1997);17:5833-42.
Analysis of transcription factor-mediated remodeling of nucleosomal arrays in a purified system.Steger DJ, Owen-Hughes T, John S,
Workman JL.
Methods (1997);12:276-85.
Stable co-occupancy of transcription factors and histones at the HIV-1 enhancer.Steger DJ, Workman JL.
EMBO J (1997);16:2463-72.
SWI/SNF stimulates the formation of disparate activator-nucleosome complexes but is partially redundant with cooperative binding.
Utley RT, Cote J, Owen-Hughes T,
Workman JL.
J Biol Chem (1997);272:12642-9. PMID:9139720
Repression of GCN5 histone acetyltransferase activity via bromodomain-mediated binding and phosphorylation by the Ku-DNA-dependent protein kinase complex.Barlev NA, Poltoratsky V, Owen-Hughes T, Ying C, Liu L,
Workman JL, Berger SL.
Mol Cell Biol (1998);18:1349-58.
Perturbation of nucleosome core structure by the SWI/SNF complex persists after its detachment, enhancing subsequent transcription factor binding.
Cote J, Peterson CL,
Workman JL.
Proc Natl Acad Sci U S A (1998);95:4947-52.
Identification and analysis of yeast nucleosomal histone acetyltransferase complexes.Eberharter A, John S, Grant PA, Utley RT,
Workman JL.
Methods (1998);15:315-21.
A subset of TAF(II)s are integral components of the SAGA complex required for nucleosome acetylation and transcriptional stimulation.
Grant PA, Schieltz D, Pray-Grant MG, Steger DJ, Reese JC, Yates JR, 3rd,
Workman JL.
Cell (1998);94:45-53. PMID:9674426
The ATM-related cofactor Tra1 is a component of the purified SAGA complex. Grant PA, Schieltz D, Pray-Grant MG, Yates JR, 3rd,
Workman JL.
Mol Cell (1998);2:863-7.
The SAGA unfolds: convergence of transcription regulators in chromatin-modifying complexes.Grant PA, Sterner DE, Duggan LJ, Workman JL, Berger SL.
Trends Cell Biol (1998);8:193-7. PMID:9695838
Transcription. A lesson in sharing?Grant PA,
Workman JL.
Nature (1998);396:410-1.
Just the facts of chromatin transcription.John S,
Workman JL.
Science (1998);282:1836-7. PMID:9874634
Bookmarking genes for activation in condensed mitotic chromosomes.John S,
Workman JL.
Bioessays (1998);20:275-9.
Persistent interactions of core histone tails with nucleosomal DNA following acetylation and transcription factor binding.
Mutskov V, Gerber D, Angelov D, Ausio J,
Workman JL, Dimitrov S.
Mol Cell Biol (1998);18:6293-304.
Purified histone acetyltransferase complexes stimulate HIV-1 transcription from preassembled nucleosomal arrays. Steger DJ, Eberharter A, John S, Grant PA,
Workman JL.
Proc Natl Acad Sci U S A (1998);95:12924-9.
Regulation of transcription by multisubunit complexes that alter nucleosome structure. Steger DJ, Utley RT, Grant PA, John S, Eberharter A, Cote J, Owen-Hughes T, Ikeda K,
Workman JL.
Cold Spring Harb Symp Quant Biol (1998);63: 483-91. PMID:10384312
Methods: a comparative to methods in enzymology.
Workman JL. Editorial. (1998);15:265-67.
Transcriptional activators direct histone acetyltransferase complexes to nucleosomes.Utley RT, Ikeda K, Grant PA, Cote J, Steger DJ, Eberharter A, John S,
Workman JL.
Nature (1998);394:498-502.
Location and function of linker histones.Vignali M,
Workman JL.
Nat Struct Biol (1998);5:1025-8.
Alteration of nucleosome structure as a mechanism of transcriptional regulation.
Workman JL, Kingston RE.
Annu Rev Biochem (1998);67:545-79.
NuA4, an essential transcription adaptor/histone H4 acetyltransferase complex containing Esa1p and the ATM-related cofactor Tra1p.
Allard S, Utley RT, Savard J, Clarke A, Grant P, Brandl CJ, Pillus L,
Workman JL, Cote J.
EMBO J (1999);18:5108-19.
Analysis of activity and regulation of hGcn5, a human histone acetyltransferase. Barlev NA,
Workman JL, Berger SL.
Methods Enzymol (1999);304:696-715. PMID:10372391
The SWI/SNF complex creates loop domains in DNA and polynucleosome arrays and can disrupt DNA-histone contacts within these domains.
Bazett-Jones DP, Cote J, Landel CC, Peterson CL,
Workman JL.
Mol Cell Biol (1999);19:1470-8.
The ADA complex is a distinct histone acetyltransferase complex in Saccharomyces cerevisiae.Eberharter A, Sterner DE, Schieltz D, Hassan A, Yates JR, 3rd, Berger SL,
Workman JL.
Mol Cell Biol (1999);19:6621-31.
Identification and analysis of native nucleosomal histone acetyltransferase complexes.Grant PA, Berger SL,
Workman JL.
Methods Mol Biol (1999);119:311-7.
Expanded lysine acetylation specificity of Gcn5 in native complexes.Grant PA, Eberharter A, John S, Cook RG, Turner BM,
Workman JL.
J Biol Chem (1999);274:5895-900. PMID:10026213
Histone acetyltransferase complexes and their link to transcription.Howe L, Brown CE, Lechner T,
Workman JL.
Crit Rev Eukaryot Gene Expr (1999);9:231-43. PMID:10651240
Activation domain-specific and general transcription stimulation by native histone acetyltransferase complexes.Ikeda K, Steger DJ, Eberharter A,
Workman JL.
Mol Cell Biol (1999);19:855-63.
A conserved motif present in a class of helix-loop-helix proteins activates transcription by direct recruitment of the SAGA complex.
Massari ME, Grant PA, Pray-Grant MG, Berger SL,
Workman JL, Murre C.
Mol Cell (1999);4:63-73. PMID:10445028
Activation domain-mediated targeting of the SWI/SNF complex to promoters stimulates transcription from nucleosome arrays.
Neely KE, Hassan AH, Wallberg AE, Steger DJ, Cairns BR, Wright AP,
Workman JL.
Mol Cell (1999);4:649-55. PMID:10549297
A novel H2A/H4 nucleosomal histone acetyltransferase in Tetrahymena thermophila.Ohba R, Steger DJ, Brownell JE, Mizzen CA, Cook RG, Cote J,
Workman JL, Allis CD.
Mol Cell Biol (1999);19:2061-8.
Analysis of nucleosome disruption by ATP-driven chromatin remodeling complexes. Owen-Hughes T, Utley RT, Steger DJ, West JM, John S, Cote J, Havas KM,
Workman JL.
Methods Mol Biol (1999);119:319-31.
Transcriptional analysis of purified histone acetyltransferase complexes.Steger DJ,
Workman JL.
Methods (1999);19:410-6.
Functional organization of the yeast SAGA complex: distinct components involved in structural integrity, nucleosome acetylation, and TATA-binding protein interaction. Sterner DE, Grant PA, Roberts SM, Duggan LJ, Belotserkovskaya R, Pacella LA, Winston F,
Workman JL, Berger SL.
Mol Cell Biol (1999);19:86-98.
Histone acetyltransferase complexes can mediate transcriptional activation by the major glucocorticoid receptor activation domain.
Wallberg AE, Neely KE, Gustafsson JA,
Workman JL, Wright AP, Grant PA.
Mol Cell Biol (1999);19:5952-9.
Function and composition of transcription adaptor/histone acetyltransferase complexes.
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Transcription regulation in eukaroyotes. Chambon P, Fukasawa T, Kornberg RD, Coath C. Eds. Human Frontier Science Program, Strasbourg, France. (1999).
Nucleosome mobilization catalysed by the yeast SWI/SNF complex.Whitehouse I, Flaus A, Cairns BR, White MF,
Workman JL, Owen-Hughes T.
Nature (1999);400:784-7.
Chromosomal gene expression.
Workman JL. Book review.
Cell (1999);96:487-9.
Gene regulation and cancer. Gene regulation and cancer: 10th anniversary meeting of the American Association of Cancer Research Special Conferences in Cancer Research, the Homestead, Hot Springs, Virginia, USA, 14-18 October 1998.
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Trends Genet (1999);15:9-10. PMID:10087926
1980s
Nucleoprotein hybridization: a method for isolating specific genes as high molecular weight chromatin.
Workman JL, Langmore JP.
Biochemistry (1985);24:7486-97. PMID:3002454
Efficient solubilization and partial purification of sea urchin histone genes as chromatin.
Workman JL, Langmore JP.
Biochemistry (1985);24:4731-8.
Isolation of specific genes as chromatin.
Workman JL. PhD Thesis. University of Michigan. 1985.
Morphometric analysis of in vivo development of porcine ovarian granulosa cells in preovulatory antral follicles. Rigby BW,
Workman JL, McLean M,, Hanzely L, Ledwitz-Rigby F.
Cytobios (1986);45:17-24.
Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II.
Workman JL, Roeder RG.
Cell (1987);51:613-22.
The pseudorabies immediate early protein stimulates in vitro transcription by facilitating TFIID: promoter interactions.
Abmayr SM,
Workman JL, Roeder RG.
Genes Dev (1988);2:542-53.
Transcriptional regulation by the immediate early protein of pseudorabies virus during in vitro nucleosome assembly.
Workman JL, Abmayr SM, Cromlish WA, Roeder RG.
Cell (1988);55:211-9.
Transcriptionally active immediate-early protein of pseudorabies virus binds to specific sites on class II gene promoters.
Cromlish WA, Abmayr SM,
Workman JL, Horikoshi M, Roeder RG.
J Virol (1989);63:1869-76.
